Deglycosylation-assisted glycoprotein de novo sequencing
downstream-application
Deglycosylation-assisted glycoprotein de novo sequencing: downstream-application. Integrative strategy for glycoprotein primary structure: enzymatic N-/O- deglycosylation to open up sequence coverage, EThcD to yield long peptides, PEAKS AB de novo…
Integrative strategy for glycoprotein primary structure: enzymatic N-/O- deglycosylation to open up sequence coverage, EThcD to yield long peptides, PEAKS AB de novo sequencing to assemble the backbone, then glycosylation site and glycan characterisation on top. Demonstrated on Etanercept and three TNFR:Fc-fusion biologics of unknown sequence.
| Kind | downstream-application |
| Deep learning | no |
| Acquisition | DDA |
| Application area | Glycoproteomics |
Data
What the papers describing this method trained it on and reported its results on, as each paper states it. A version is named only where the paper names one.
Also used, purpose not recorded
Paper describing it
- Decoding Protein Glycosylation by an Integrative Mass Spectrometry-Based De Novo Sequencing Strategy (2025, JACS Au, peer-reviewed)