Multi-enzyme template-assisted mAb sequencing
downstream-application
Multi-enzyme template-assisted mAb sequencing: downstream-application. Complete monoclonal-antibody sequencing from multiple enzyme digests read by HCD and EThcD, with the de novo calls made template-assisted in Supernovo. Refined on an…
Complete monoclonal-antibody sequencing from multiple enzyme digests read by HCD and EThcD, with the de novo calls made template-assisted in Supernovo. Refined on an antibody of known sequence, then applied to 25 anti-haemagglutinin influenza antibodies of unknown sequence, recovering high-confidence sequence for over 99% of the CDRs; the resulting heavy and light chains were cloned and expressed, and the recombinant antibodies reproduced the original binding curves. Distinguishes Ile from Leu correctly on the known-sequence controls.
| Kind | downstream-application |
| Deep learning | no |
| Acquisition | DDA |
| Application area | Antibodyomics |
Paper describing it
- Template-Assisted De Novo Sequencing of SARS-CoV-2 and Influenza Monoclonal Antibodies by Mass Spectrometry (2022, Journal of Proteome Research, peer-reviewed)