A kind of method for determining antibodies in blood amino acid sequence
CN107525934A · Individual · priority 2016
| Patent | CN107525934A ↗ |
| Status | Pending |
| Priority date | 2016-06-21 |
| Filed | 2016-06-21 |
| Published | 2017-12-29 |
| Assignee | Individual |
Abstract
A kind of method for determining antibodies in blood amino acid sequence, the purpose is to the amino acid sequence of human or animal’s antibodies in blood is directly determined by biochemical method.This method including the step of be：A, the specific antibody combined with specific antigen is separated from blood or blood product；B, the specific antibody of separation is produced into antibody fragment using protease digestion；C, obtained antibody fragment is separated using SDS PAGE electrophoresis with ion-exchange chromatography, positive chromatogram, reverse chromatograms, two dimensional electrophoresis or isoelectric focusing electrophoresis, obtains the component for being no more than five different aminoacids sequence antibodies fragments；D, the antibody fragment component of separation is cut into the peptide fragment of 5 to 60 amino acid；E, the amino acid sequence of peptide fragment obtained by determining；Antibody sequence is spliced into by lap with F, by the peptide fragment amino acid sequence of measure.
Why it is here
Zhang Xiaolong: sequencing antigen-specific antibodies from blood by multi-protease digestion, peptide sequencing by mass spectrometry and overlap assembly.
Inventors (2)
张效龙, 金茜
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Cited by (2 patent families, 2 organisations)
- 中外制药株式会社 company: CN115380210A
- 杭州中晟纳博生物科技有限公司 company: CN115044983A
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