Antibody sequencing method and system based on mass spectrum and application thereof
CN119040445A · Beijing Bio-Tech Pack Technology · priority 2024
| Patent | CN119040445A ↗ |
| Status | Pending |
| Priority date | 2024-08-21 |
| Filed | 2024-08-21 |
| Published | 2024-11-29 |
| Assignee | Beijing Bio-Tech Pack Technology (printed Beijing Biotech Biotechnology Co ltd) |
Abstract
Translated from Chinese 本发明属于氨基酸检测分析技术领域，具体涉及一种基于质谱的抗体测序方法、系统及其应用。具体的，本发明采用多种酶组合进行胶内酶切与溶液酶切，得到具有不同程度重叠度的肽段，保证抗体序列的全长覆盖，进而可用于解析数据库中不包含的或者序列不公开的蛋白序列，以及发生不同程度突变的蛋白序列，从而实现对未知抗体(蛋白)的序列测定，该测序方法具有良好的准确度，具有良好的实际应用之价值。 The present invention belongs to the technical field of amino acid detection and analysis, and specifically relates to a mass spectrometry-based antibody sequencing method, system and application thereof. Specifically, the present invention uses a combination of multiple enzymes for in-gel digestion and solution digestion to obtain peptides with different degrees of overlap, ensuring full-length coverage of the antibody sequence, and can be used to parse protein sequences that are not included in the database or whose sequences are not public, as well as protein sequences with different degrees of mutation, thereby realizing the sequence determination of unknown antibodies (proteins). The sequencing method has good accuracy and good practical application value.
Why it is here
Beijing Biotech: antibody sequencing by de novo sequencing of peptides from MS2 and MS3 spectra, then splicing.
Inventors (4)
王旭, 李龙, 周珊珊, 邢颖
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