Antibody sequencing methods and devices
CN121281629A · Xiang An Biomedicine Laboratory · priority 2024
| Patent | CN121281629A ↗ |
| Status | Pending |
| Priority date | 2024-07-04 |
| Filed | 2024-07-04 |
| Published | 2026-01-06 |
| Assignee | Xiang An Biomedicine Laboratory (printed Xiang’an Innovation Laboratory; Xiamen University) |
Abstract
Translated from Chinese 本发明属于生物分析技术领域，涉及一种抗体测序方法和装置，具体地，涉及一种基于束搜索的抗体肽序列组装的方法和装置。具体地，本发明涉及一种抗体测序方法，包括如下步骤：S1，获得待测抗体的同源模板；S2，对肽段从头测序数据进行清洗，得到清洗后的肽段序列；S3，将清洗后的肽段序列分割成固定长度的短肽序列；S4，基于同源模板上的氨基酸信息和短肽序列的信号强度，通过束搜索构建德布鲁因图，并进行序列组装，得到抗体序列。本发明能够提高单克隆抗体从头测序的通量，从而降低质谱检测时间和实验耗材成本，具有良好的应用前景。 This invention belongs to the field of bioanalytical technology and relates to an antibody sequencing method and apparatus, specifically, a method and apparatus for assembling antibody peptide sequences based on beam search. Specifically, this invention relates to an antibody sequencing method comprising the following steps: S1, obtaining a homologous template for the antibody to be tested; S2, cleaning the de novo peptide sequencing data to obtain cleaned peptide sequences; S3, segmenting the cleaned peptide sequences into short peptide sequences of fixed length; S4, constructing a de Bruin diagram based on the amino acid information on the homologous template and the signal intensity of the short peptide sequences using beam search, and assembling the sequences to obtain the antibody sequences. This invention can improve the throughput of de novo sequencing of monoclonal antibodies, thereby reducing mass spectrometry detection time and experimental consumable costs, and has good application prospects.
Why it is here
Xiang’an Innovation Laboratory: antibody sequencing by assembling cleaned de novo peptide sequences on a homologous template with a de Bruijn graph.
Inventors (6)
江文彬, 俞容山, 熊悦婷, 肖瑾, 袁权, 夏宁邵
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