Amino acid sequence analysis system using mass spectrometry
JP2007278712A · Shimadzu (Japan) · priority 2006
| Patent | JP2007278712A ↗ |
| Status | Granted |
| Priority date | 2006-04-03 |
| Filed | 2006-04-03 |
| Published | 2007-10-25 |
| Assignee | Shimadzu (Japan) (printed Shimadzu Corp) |
| Also published as | JP4857872B2 |
Abstract
Translated from Japanese 【課題】デノボ・シーケンスを用いたペプチドのアミノ酸配列の推定精度を高める。 【解決手段】ペプチド混合物についてのＭＳ ２ 分析とＭＳ ３ 分析を実行しそれぞれマススペクトルを取得する（Ｓ１、Ｓ２）。それから、両マススペクトルに共通に現れるピークを集めたピークリスト（Ｓ３）、ＭＳ ２ ／ＭＳ ３ 分析のプリカーサの質量数差だけＭＳ ３ マススペクトルを高質量数側にシフトして作成したスペクトルとＭＳ ２ マススペクトルとに共通に現れるピークを集めたピークリスト（Ｓ４）、など全部で６種類の、各末端別のピークを集めたピークリストを作成し（Ｓ５〜Ｓ８）、その後にＮ末端、Ｃ末端別のピークリストに集約する（Ｓ９）。この２つのピークリストをデノボ・シーケンスの解析用ソフトウエアに供しアミノ酸配列推定を実行する。推定結果の信頼度が低い場合にはＳ２に戻り別のプリカーサを選択してＭＳ ３ 分析を実行しＳ３〜Ｓ１０を繰り返す。 【選択図】図２ An object of the present invention is to improve the estimation accuracy of amino acid sequences of peptides using a de novo sequence. MS 2 analysis and MS 3 analysis are performed on a peptide mixture, and mass spectra are acquired respectively (S1, S2). Then, a peak list (S3) that collects peaks that appear in both mass spectra, a spectrum created by shifting the MS 3 mass spectrum to the high mass number side by the mass number difference of the precursor of MS 2 / MS 3 analysis, and MS A peak list (S5 to S8) in which a total of six types of peaks are collected (S4 to S8), which collect peaks commonly appearing in the two mass spectra, etc. The peaks are classified into terminal-specific peak lists (S9). The two peak lists are provided to de novo sequence analysis software to execute amino acid sequence estimation. When the reliability of the estimation result is low, the process returns to S2, selects another precursor, performs MS 3 analysis, and repeats S3 to S10. [Selection] Figure 2
Why it is here
Shimadzu: amino acid sequence estimation from peaks shared between MSn and MSn+1 spectra, collected per terminus.
Inventors (6)
Shigeki Kajiwara, 茂樹 梶原, Shinichi Iwamoto, 慎一 岩本, Koichi Tanaka, 耕一 田中
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Cited by (4 patent families, 1 organisations)
- Shimadzu company, its own earlier patent: WO2009081446A1, JP2012251878A, JP2013088377A, JP2013160595A
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.