Method for determining amino acid sequence of peptide using mass spectrometry, peptide derivatization reagent used in the method, and reagent kit
JP2008196965A · Kyoto University · priority 2007
| Patent | JP2008196965A ↗ |
| Status | Pending |
| Priority date | 2007-02-13 |
| Filed | 2007-02-13 |
| Published | 2008-08-28 |
| Assignee | Kyoto University |
Abstract
Translated from Japanese 【課題】本発明の目的は、質量分析法により、簡便且つ効率的にペプチドのアミノ酸配列を完全に決定できる方法、及び当該方法に使用されるペプチド誘導体化試薬及び試薬キットを提供することである。 【解決手段】質量分析法を用いたペプチドのアミノ酸配列を決定する方法において、(1)ペプチドのN末端アミノ基に下記一般式(I)で示す基を結合させることによりペプチドを誘導体化する工程、(2)前記工程(1)で誘導体化されたペプチドに対して、フラグメントイオンの質量スペクトルを取得する工程、及び(3)前記工程(2)で得られた質量スペクトルに基づいて、ペプチドのアミノ酸配列を決定する工程を順次実施する。 【選択図】なし An object of the present invention is to provide a method capable of completely and efficiently determining the amino acid sequence of a peptide by mass spectrometry, and a peptide derivatization reagent and a reagent kit used in the method. . In a method for determining the amino acid sequence of a peptide using mass spectrometry, (1) a step of derivatizing the peptide by binding a group represented by the following general formula (I) to the N-terminal amino group of the peptide (2) obtaining a fragment ion mass spectrum for the peptide derivatized in the step (1), and (3) based on the mass spectrum obtained in the step (2), The step of determining the amino acid sequence is sequentially performed. [Selection figure] None
Why it is here
Kyoto University: amino acid sequence determined from the fragment spectra of N-terminally derivatised peptides.
Inventors (6)
Masahiro Miyashita, 正弘 宮下, Yukari Otake, 由香里 大竹, Yosuke Hanai, 陽介 花井
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Cited by (1 patent families, 1 organisations)
- K.I.C.A company: CN102264257A
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