Precise de novo sequencing method for top-down proteomics
US20260188427A1 · Florida State University · priority 2024
| Patent | US20260188427A1 ↗ |
| Status | Pending |
| Priority date | 2024-08-26 |
| Filed | 2026-02-20 |
| Published | 2026-07-02 |
| Assignee | Florida State University (printed Florida State University Research Foundation Inc) |
| Also published as | US20260057966A1, WO2026050267A1 |
Abstract
Computerized methods and systems of de novo sequencing from a mass spectrometer and identifying a biological polymer using mass invariant charge patterns in the spectrometer data by transforming spectra to a natural logarithmic space where peaks arising from the same analyte mass align along a predictable pattern defined solely by charge state. In some embodiments, the computerized method employs an operation that iterates the residue mass in the transformed natural logarithmic space, e.g., minimizing charge state difference errors between corresponding isotopologues assigned to different charge states. In some embodiments, the de novo sequencing of the present disclosure also allows for viewing the mass-to-charge (m/z) spectrum in a natural logarithmic manner (e.g., Equation 1 −ln(m/z−q)) to provide confidence in any reassignment of peaks in an observed charge pattern vector.
Why it is here
Florida State University: de novo sequencing of top-down spectra after transforming m/z values to charge-invariant values.
Inventors (1)
Lissa Caitlin Anderson
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