Methods of identifying peptides and proteins by mass spectrometry

US6489121B1 · Micromass UK · priority 1999

Patent US6489121B1 (Micromass UK, Expired - Lifetime): A method of identifying a protein, polypeptide or peptide by means of mass spectrometry and especially by tandem mass spectrometry is disclosed. The method preferably models the fragmentation of…
Patent US6489121B1 ↗
Status Expired - Lifetime
Priority date 1999-04-06
Filed 2000-04-06
Published (granted) 2002-12-03
Assignee Micromass UK (printed Micromass UK Ltd)
Also published as CA2303758A1, CA2303758C, DE60031030D1, DE60031030T2, EP1047108A2, EP1047108A3, EP1047108B1, US20030028932A1, US20060247865A1, US7069152B2

Abstract

A method of identifying a protein, polypeptide or peptide by means of mass spectrometry and especially by tandem mass spectrometry is disclosed. The method preferably models the fragmentation of a peptide or protein in a tandem mass spectrometer to facilitate comparison with an experimentally determined spectrum. A fragmentation model is used which takes account of all possible fragmentation pathways which a particular sequence of amino acids may undergo. A peptide or protein may be identified by comparing an experimentally determined mass spectrum with spectra predicted using such a fragmentation model from a library of known peptides or proteins. Alternatively, a de novo method of determining the amino acid sequence of an unknown peptide using such a fragmentation model may be used.

Why it is here

Micromass (Skilling): Bayesian likelihood of trial sequences over fragmentation routes; dependent claims draw the trial sequences at random rather than from a database.

Inventors (1)

John Skilling

An inventor links to an author page only where the same person is evident, never on a name alone.

Catalogued patents it cites (1)

  • US6582965B1 Method for de novo peptide sequence determination

Cited by (20 patent families, 18 organisations)

Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.

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