Method of determining peptide sequences by mass spectrometry

US6489608B1 · Micromass UK · priority 1999

Patent US6489608B1 (Micromass UK, Expired - Lifetime): A method of determining the sequence of amino acids that constitute peptides, polypeptides or proteins by mass spectrometry and especially by tandem mass spectrometry is disclosed without the…
Patent US6489608B1 ↗
Status Expired - Lifetime
Priority date 1999-04-06
Filed 2000-04-06
Published (granted) 2002-12-03
Assignee Micromass UK (printed Micromass UK Ltd)
Also published as CA2303761A1, CA2303761C, DE60026452D1, DE60026452T2, EP1047107A2, EP1047107A3, EP1047107B1, EP1688987A1

Abstract

A method of determining the sequence of amino acids that constitute peptides, polypeptides or proteins by mass spectrometry and especially by tandem mass spectrometry is disclosed without the use of any additional data concerning the nature of the peptide and without any limit to the number of possible sequences considered. The method can be implemented on a personal computer typically used for data acquisition on the tandem mass spectrometer even in the case of peptides comprising 10 or more amino acids. The method does not rely on exhaustive comparison of the spectra predicted from every possible amino acid sequence with any molecular weight constraint, but instead uses mathematical techniques to simulate the effect of such a complete search without actually carrying it out.

Why it is here

Micromass (Skilling): de novo sequencing by a terminated Markov chain Monte Carlo search over trial sequences drawn from a prior.

Inventors (1)

John Skilling

An inventor links to an author page only where the same person is evident, never on a name alone.

Catalogued patents it cites (1)

  • US6582965B1 Method for de novo peptide sequence determination

Cited by (20 patent families, 18 organisations)

Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.

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