Sequencing of peptides by mass spectrometry
US6799121B2 · York University · priority 2000
| Patent | US6799121B2 ↗ |
| Status | Expired - Fee Related |
| Priority date | 2000-03-30 |
| Filed | 2001-03-13 |
| Published (granted) | 2004-09-28 |
| Assignee | York University |
| Also published as | US20020001814A1 |
Abstract
A strategy for semiautomatic sequencing of argentinated (silver-containing) oligopeptides is described. The method of sequencing described is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M+Ag]<+ >ion of an oligopeptide. The ions that constitute a triplet are [bn+OH+Ag]<+>, [bn-H+Ag]<+>, and [an-H+Ag]<+>, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is “cleaved”. Observation of the [yn+H+Ag]<+ >ion containing the cleaved residue confirms the assignment.
Why it is here
York University (Siu): partial sequences of argentinated peptides read from silver-containing doublet and triplet patterns.
Inventors (3)
Ivan K. Chu, Tai-Chu Lau, K. W. Michael Siu
An inventor links to an author page only where the same person is evident, never on a name alone.
Cited by (2 patent families, 2 organisations)
- Thermo Finnigan company: US7595485B1
- アプレラ コーポレイション company: JP4160558B2
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.