Method of de novo sequencing of peptide using the MALDI mass spectrometry, method and apparatus for preparing sample for MALDI mass spectrometry
US7723917B2 · Pohang University of Science and Technology · priority 2005
| Patent | US7723917B2 ↗ |
| Status | Active |
| Priority date | 2005-03-08 |
| Filed | 2006-03-08 |
| Published (granted) | 2010-05-25 |
| Assignee | Pohang University of Science and Technology (printed Posco Co Ltd; Pohang University of Science and Technology Foundation; POSTECH Academy-Industry Foundation) |
| Current assignee | Pohang University of Science and Technology Foundation; POSTECH Academy-Industry Foundation; Posco Holdings Inc |
| Also published as | ATE508208T1, DE602006021733D1, EP1871898A1, EP1871898A4, EP1871898B1, EP2103945A1, EP2103945B1, US20080138845A1, WO2006096012A1 |
Abstract
The present invention relates to a method of peptide sequencing by MALDI (matrix-assisted laser desorption ionization) tandem mass spectrometry, which comprises the steps of chemically modifying a sample peptide with at least one chemical modification method selected from the group consisting of guanidination and esterification in order to change the ionization status of the peptide and performing mass spectrometry using a MALDI tandem mass spectrometer and programmed MALDI tandem mass spectrometry. The peptide sequencing method of the present invention is advantageous in that detection sensitivity of the peptide improves significantly and various daughter ions are detected uniformly, thereby enabling perfect de novo sequencing with tandem mass spectrometry only, without database search.
Why it is here
POSTECH: MALDI tandem MS de novo sequencing of guanidinated and esterified peptides.
Inventors (5)
Seung-Koo Shin, Kyung-Hwan Jeong, Hye-Joo Yoon, Min-Soo Suh, Jong-Cheol Seo
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued papers it cites (2)
- Derivatization procedures to facilitate de novo sequencing of lysine-terminated tryptic peptides using postsource decay matrix-assisted laser desorption/ionization mass spectrometry (2000)
- Highly informative proteome analysis by combining improved N ‐terminal sulfonation for de novo peptide sequencing and online capillary reverse‐phase liquid chromatography/tandem mass spectrometry (2004)
Matched from the patent’s 10 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- US6829539B2 Methods for quantification and de novo polypeptide sequencing by mass spectrometry
Cited by catalogued patents (1)
- CN106645437B De novo sequencing method based on chemically modified and isotopically labeled polypeptide amino acid sequences
Cited by (4 patent families, 3 organisations)
- Shimadzu company: JP5874587B2, JP6136771B2
- 中国科学院大连化学物理研究所 academic: CN106645437B
- 浜松ホトニクス株式会社 company: JP6743224B1
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.