Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry
US8119411B2 · NEC · priority 2002
| Patent | US8119411B2 ↗ |
| Status | Expired - Fee Related |
| Priority date | 2002-12-26 |
| Filed | 2010-12-20 |
| Published (granted) | 2012-02-21 |
| Assignee | NEC (printed NEC Corp) |
| Also published as | AU2003292621A1, EP1577672A1, EP1577672A4, JP2004251623A, JP3534191B1, US20060134720A1, US20110183428A1, US7879616B2, WO2004059323A1 |
Abstract
The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.
Why it is here
NEC: C-terminal sequence read from the mass differences of a ladder made by successive chemical release of C-terminal residues.
Inventors (4)
Kenji Miyazaki, Akira Tsugita, Kenichi Kamijo, Hiroaki Torii
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued patents it cites (1)
- US6582965B1 Method for de novo peptide sequence determination
Cited by (10 patent families, 9 organisations)
- DH Technologies Development company: US20130204582A1, WO2012035412A2
- Nec company, its own earlier patent: EP1983346A4
- Shimadzu company: WO2014162557A1
- Sidebar company: AU2006238849A1
- 日本电气株式会社 company: CN101379074A
- 武汉迈维代谢生物科技股份有限公司 company: CN121027273B
- 한국과학기술연구원 company: KR102093818B1
- 한국표준과학연구원 company: KR100963539B1
- Ｎｅｃソフト株式会社 company: JP4555256B2
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.