Peptide sequencing using mass spectrometry

WO1994006017A1 · Selectide Corporation · priority 1992

Patent WO1994006017A1 (Selectide Corporation, Ceased): The present invention relates to methods for determining the amino acid composition, and more preferably the sequence, of a peptide using mass spectrometric techniques. The method is…
Patent WO1994006017A1 ↗
Status Ceased
Priority date 1992-09-03
Filed 1993-09-02
Published 1994-03-17
Assignee Selectide Corporation (printed Selectide Corp)
Also published as AU4844893A, US5470753A

Abstract

The present invention relates to methods for determining the amino acid composition, and more preferably the sequence, of a peptide using mass spectrometric techniques. The method is particularly useful for sequencing peptides isolated from natural sources or from libraries of peptides that have been prepared synthetically, and for peptides that are not amenable to Edman degradation sequencing. In one embodiment, the method for determining the amino acid composition or sequence of a peptide comprises determining the difference of the mass of the peptide from the mass of a deuterium-hydrogen exchanged peptide, and from this difference determining the number of exchangeable (labile) hydrogen atoms (protons). Candidate peptides having amino acid compositions or sequences that do not contain the observed number of exchangeable protons are eliminated. In another embodiment, synthesis of a portion of the peptides in a library of peptides is terminated after each coupling step, whereby a set of sequentially truncated fragments of each peptide is formed. Thus the sequence of the peptide can be determined by determining the difference in the molecular weight between each peptide or fragment thereof and the sequentially smaller fragment of the peptide.

Why it is here

Selectide: candidate sequences from mass spectrometric analysis pruned by counting reactive groups or exchangeable protons.

Inventors (4)

Nikolai Sepetov, Olga Issakova, Viktor Krehnak, Michal Lebl

An inventor links to an author page only where the same person is evident, never on a name alone.

Cited by catalogued patents (1)

  • US6271037B1 Method and product for the sequence determination of peptides using a mass spectrometer

Cited by (45 patent families, 33 organisations)

Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.

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