Direct-read precision de novo sequencing method for proteins and proteome and based on product ion type identification and full product-ion coverage
WO2025156912A1 · Academy of Military Medical Sciences · priority 2024
| Patent | WO2025156912A1 ↗ |
| Status | Pending |
| Priority date | 2024-01-22 |
| Filed | 2024-12-25 |
| Published | 2025-07-31 |
| Assignee | Academy of Military Medical Sciences |
| Claims | DiNovo |
| Also published as | CN118033144A |
Abstract
A direct-read precision de novo sequencing method for amino acid sequences of proteins and proteome, the method involving determining the type of product ions on the basis of lysine (K) and arginine (R) dual mass-defect amino acid encoding (NeuCode) labeling, and performing paired mirror digestion to achieve the direct-read precision de novo sequencing of animo acid sequences of proteins with full product-ion coverage. A protein is labeled with K/R NeuCode amino acids, mirror peptide fragments are obtained by means of paired mirror digestion, and the mirror peptide fragments are subjected to tandem mass spectrometry analysis to obtain a mirror spectrum with full product-ion coverage and product ion type identification. Consequently, the amino acid sequence information in a secondary spectrum can be directly read on the basis of the mass difference of two adjacent product ions of the same type, thereby realizing direct reading and precision sequencing of the amino acid sequence of the protein.
Why it is here
Academy of Military Medical Sciences: direct-read de novo sequencing with mirror proteases and mass-defect labelled lysine and arginine; the inventors are DiNovo’s authors.
Inventors (7)
Ping Xu, Li Kang, Yanchang Li, Jiaxing Dai, Yao Zhang, 姜松昊, 常蕾
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued papers it cites (1)
- Neutron-encoded Signatures Enable Product Ion Annotation From Tandem Mass Spectra (2013) added by the examiner
Matched from the patent’s 4 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- CN105301119B De novo sequencing of protein amino acid sequences based on non-isobaric markers at both ends
Cited by (1 patent families, 1 organisations)
- 中国人民解放军军事科学院军事医学研究院 academic: CN118033144A
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.