An improved method for de novo sequencing of arginine‐containing, N α ‐tris(2,4,6‐trimethoxyphenyl)phosphonium‐acetylated peptides
peer-reviewed · Rapid Communications in Mass Spectrometry · 2008
peer-reviewed · Rapid Communications in Mass Spectrometry · 2008. Hiroki Kuyama et al. An improved method for de novo sequencing of arginine-containing peptides modified with…
| Date | 2008-07-15 |
| Type | peer-reviewed |
| Venue | Rapid Communications in Mass Spectrometry |
| Publisher | Wiley |
| Contribution | algorithm |
| DOI | 10.1002/rcm.3587 |
| Citations (OpenAlex) | 16 |
| Venue 2-year citedness | 1.80 |
Abstract
An improved method for de novo sequencing of arginine-containing peptides modified with succinimidyloxycarbonylmethyl tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP-Ac-OSu) is reported. A tagging reagent, TMPP-Ac-OSu, was introduced to improve the sequence analysis of peptides owing to the simplified fragmentation pattern. However, peptides containing arginine residues did not fragment efficiently even after TMPP-Ac modification at their N-termini. This report describes how fragmentation efficiency of TMPP-Ac-modified arginine-containing peptides was significantly improved by modifying the guanidino group on the side chain of arginine with acetylacetone.
Methods and tools
- TMPP arginine-peptide de novo sequencing: Extends TMPP-Ac-OSu tagging to arginine-containing peptides, whose fragmentation the basic side chain otherwise complicates, simplifying the pattern enough to read a sequence.