DPST: De Novo Peptide Sequencing with Amino-Acid-Aware Transformers
preprint · arXiv · 2022
| Date | 2022-03-23 |
| Type | preprint |
| Venue | arXiv |
| Publisher | arXiv |
| Contribution | algorithm |
| DOI | 10.48550/arXiv.2203.13132 |
| Citations (OpenAlex) | 8 |
Abstract
De novo peptide sequencing aims to recover amino acid sequences of a peptide from tandem mass spectrometry (MS) data. Existing approaches for de novo analysis enumerate MS evidence for all amino acid classes during inference. It leads to over-trimming on receptive fields of MS data and restricts MS evidence associated with following undecoded amino acids. Our approach, DPST, circumvents these limitations with two key components: (1) A confidence value aggregation encoder to sketch spectrum representations according to amino-acid-based connectivity among MS; (2) A global-local fusion decoder to progressively assimilate contextualized spectrum representations with a predefined preconception of localized MS evidence and amino acid priors. Our components originate from a closed-form solution and selectively attend to informative amino-acid-aware MS representations. Through extensive empirical studies, we demonstrate the superiority of DPST, showing that it outperforms state-of-the-art approaches by a margin of 12% - 19% peptide accuracy.
Methods and tools
- DPST: Amino-acid-aware transformer
Cites (5)
- Computationally instrument-resolution-independent de novo peptide sequencing for high-resolution devices (2021) semanticscholar
- Uncovering Thousands of New Peptides with Sequence-Mask-Search Hybrid De Novo Peptide Sequencing Framework (2019) semanticscholar
- De novo peptide sequencing by deep learning (2017) semanticscholar
- A Dynamic Programming Approach to De Novo Peptide Sequencing via Tandem Mass Spectrometry (2001) semanticscholar
- De novo peptide sequencing via tandem mass spectrometry (1999) semanticscholar
Cited by (11)
- AbNovoBench: a resource and benchmarking platform for monoclonal antibody de novo sequencing (2026) crossref
- A transformer model for de novo sequencing of data independent acquisition mass spectrometry data (2025) crossref
- MassNet: billion-scale AI-friendly mass spectral corpus enables robust de novo peptide sequencing (2025) crossref
- De Novo Peptide Sequencing for Data-independent Acquisition (DIA) Using Deep Learning (2025) crossref
- InstaNovo enables diffusion-powered de novo peptide sequencing in large-scale proteomics experiments (2025) both
- PepGo: a deep learning and tree search-based model for de novo peptide sequencing (2025) crossref
- Deep Learning Methods for De Novo Peptide Sequencing (2024) crossref
- A multi-species benchmark for training and validating mass spectrometry proteomics machine learning models (2024) crossref
- A transformer model for de novo sequencing of data independent acquisition mass spectrometry data (2024) crossref
- De novo peptide sequencing with InstaNovo: Accurate, database-free peptide identification for large scale proteomics experiments (2023) both
- Comprehensive evaluation of peptide de novo sequencing tools for monoclonal antibody assembly (2023) both