Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus
peer-reviewed · International Journal of Molecular Sciences · 2014
| Date | 2014-02-11 |
| Type | peer-reviewed |
| Venue | International Journal of Molecular Sciences |
| Publisher | MDPI AG |
| Contribution | downstream-application |
| DOI | 10.3390/ijms15022465 |
| Citations (OpenAlex) | 8 |
Abstract
Amino acid substitutions in influenza A virus are the main reasons for both antigenic shift and virulence change, which result from non-synonymous mutations in the viral genome. Nucleocapsid protein (NP), one of the major structural proteins of influenza virus, is responsible for regulation of viral RNA synthesis and replication. In this report we used LC-MS/MS to analyze tryptic digestion of nucleocapsid protein of influenza virus (A/Puerto Rico/8/1934 H1N1), which was isolated and purified by SDS poly-acrylamide gel electrophoresis. Thus, LC-MS/MS analyses, coupled with manual de novo sequencing, allowed the determination of three substituted amino acid residues R452K, T423A and N430T in two tryptic peptides. The obtained results provided experimental evidence that amino acid substitutions resulted from non-synonymous gene mutations could be directly characterized by mass spectrometry in proteins of RNA viruses such as influenza A virus.
Methods and tools
- Influenza nucleocapsid substitution mapping: Reads amino acid substitutions in the influenza A nucleocapsid protein by mass spectrometry with de novo sequencing, substitutions being the route by which non-synonymous mutations become antigenic shift and changed virulence.