Unveiling the Diversity and Modifications of Short Peptides in Scorpion Venom through Liquid Chromatography-High Resolution Mass Spectrometry
peer-reviewed · Toxins · 2024
| Date | 2024-03-16 |
| Type | peer-reviewed |
| Venue | Toxins |
| Publisher | MDPI AG |
| Contribution | downstream-application |
| DOI | 10.3390/toxins16030155 |
| Citations (OpenAlex) | 7 |
| Venue 2-year citedness | 4.56 |
Abstract
More recently, short peptides in scorpion venom have received much attention because of their potential for drug discovery. Although various biological effects of these short peptides have been found, their studies have been hindered by the lack of structural information especially in modifications. In this study, small peptides from scorpion venom were investigated using high-performance liquid chromatography high-resolution mass spectrometry followed by de novo sequencing. A total of 156 sequences consisting of 2~12 amino acids were temporarily identified from Buthus martensii scorpion venom. The identified peptides exhibited various post-translational modifications including N-terminal and C-terminal modifications, in which the N-benzoyl modification was first found in scorpion venom. Moreover, a short peptide Bz-ARF-NH2 demonstrated both N-terminal and C-terminal modifications simultaneously, which is extremely rare in natural peptides. In conclusion, this study provides a comprehensive insight into the diversity, modifications, and potential bioactivities of short peptides in scorpion venom.
Methods and tools
- Scorpion venom short peptides: LC-HRMS with de novo sequencing of short (2-12 aa) scorpion venom peptides: 156 sequences identified, revealing N- and C-terminal modifications including the previously unreported N-benzoyl modification.
Cites (6)
- Accurate discrimination of leucine and isoleucine residues by combining continuous digestion with multiple MS3 spectra integration in protein sequence (2022) both
- Mass spectrometric identification and denovo sequencing of novel conotoxins from vermivorous cone snail (Conus inscriptus), and preliminary screening of its venom for biological activities in vitro and in vivo (2021) both
- De novo sequencing of proteins by mass spectrometry (2020) both
- Discrimination of Leucine and Isoleucine in Peptides Sequencing with Orbitrap Fusion Mass Spectrometer (2014) both
- Moving Pieces in a Venomic Puzzle: Unveiling Post-translationally Modified Toxins from Tityus serrulatus (2013) both
- De novo sequencing of peptides by MS/MS (2010) both