Plant-pathogen effectors
4 workflows · 2004–2017
Plant-pathogen effectors: Effector proteins secreted by plant pathogens, often from organisms with no usable reference. 4 catalogued workflows and 5 papers.
Effector proteins secreted by plant pathogens, often from organisms with no usable reference.
| Workflows | 4 |
| Papers | 5 |
| Authors | 21 |
| Active | 2004-01-01 to 2017-08-01 |
Workflows (4)
- High plains disease agent characterization (2004): Downstream application of de novo peptide sequencing to an unidentified plant pathogen. The 32-kDa protein specific to high plains disease was sequenced by time-of-flight MS after the agent was isolated in pure culture by vascular puncture inoculation. De novo sequencing of peptides from proteolytic digests of the SDS-PAGE band corrected the public record: the GenBank nucleotide-derived sequence U60141, deposited as the probable N-protein of high plains virus, turned out to be incomplete, and 18 further residues were found at the N terminus. BLAST then returned no significant homology to any protein in the databases, indicating a hitherto unclassified virus group. A clean early demonstration of why de novo matters: the answer was unreachable by database search because the correct sequence was not in any database.
- Plant virus coat protein identification (2010): Identifies known and novel plant viruses from coat protein MS/MS spectra by virus database matching and de novo sequence analysis.
- Plasmopara elicitor de novo workflow (2010): Downstream application combining de novo peptide sequencing algorithms with RACE-PCR to identify a Plasmopara halstedii elicitor protein.
- Lasiodiplodia theobromae proteome (2017): Proteomics of the trunk-disease fungus Lasiodiplodia theobromae, where de novo peptide sequencing alongside database search revealed novel proteins including proteases and allergenic enolases.
Papers (5)
- Characterization of the Agent of “High Plains Disease”: Mass Spectrometry Determines the Sequence of the Disease-specific Protein (2004, Journal of Biological Chemistry, peer-reviewed)
- A generic method to identify plant viruses by high-resolution tandem mass spectrometry of their coat proteins (2010, Journal of Virological Methods, peer-reviewed)
- Identification of a novel Plasmopara halstedii elicitor protein combining de novo peptide sequencing algorithms and RACE-PCR (2010, Proteome Science, peer-reviewed)
- Novel proteins from proteomic analysis of the trunk disease fungus Lasiodiplodia theobromae (Botryosphaeriaceae) (2017, Biochimie Open, peer-reviewed)
- Data from proteome analysis of Lasiodiplodia theobromae (Botryosphaeriaceae) (2017, Data in Brief, peer-reviewed)
Where the work happened
Canada, Germany, Mexico, New Zealand, USA