351 nm UVPD de novo sequencing
adjacent · Chemical derivatization assisted
351 nm UVPD de novo sequencing: adjacent · Chemical derivatization assisted. N-terminal chromophore derivatisation makes a peptide absorb at 351 nm, so selective UV photodissociation cleaves it into a simplified y-ion series instead of the mixed…
N-terminal chromophore derivatisation makes a peptide absorb at 351 nm, so selective UV photodissociation cleaves it into a simplified y-ion series instead of the mixed ladders CID gives, deliberately to make the spectra amenable to de novo sequencing.
| Kind | adjacent |
| Family | Chemical derivatization assisted |
| Deep learning | no |
| Acquisition | DDA |
Papers
- De Novo Sequencing of Peptides Using Selective 351 nm Ultraviolet Photodissociation Mass Spectrometry (2013, Analytical Chemistry, peer-reviewed)