Chemical derivatization assisted
5 methods · 1999–2026
Chemical derivatization assisted: Covalent modification of the peptide, usually at a terminus, to change how it fragments: selective cleavage, brighter low-mass ions, or a strongly directed ion series.
Covalent modification of the peptide, usually at a terminus, to change how it fragments: selective cleavage, brighter low-mass ions, or a strongly directed ion series.
The earliest of its 5 methods is PSD MALDI high-sensitivity de novo sequencing (1999); 4 more have followed.
| Methods | 5 |
| Papers describing them | 6 |
| Authors | 33 |
| Active | 1999-06-22 to 2026-06-30 |
| Kinds | adjacent (5) |
| Acquisition | DDA (4) |
Methods (5)
Oldest first, by the paper that describes each one.
- PSD MALDI high-sensitivity de novo sequencing (1999): High-sensitivity postsource decay MALDI method for de novo peptide sequencing with N-terminal sulfonation.
- Lysine derivatization PSD de novo sequencing (2000): Derivatization workflow for lysine-terminated tryptic peptides to facilitate PSD MALDI de novo sequencing.
- 351 nm UVPD de novo sequencing (2013): N-terminal chromophore derivatisation makes a peptide absorb at 351 nm, so selective UV photodissociation cleaves it into a simplified y-ion series instead of the mixed ladders CID gives, deliberately to make the spectra amenable to de novo sequencing.
- PSMtags (2025): Small-molecule peptide conjugates found by systematic screening that sharply increase b-ion fragment coverage, raising sequence-identification confidence and improving de novo sequencing. Also supports 9-plex stable-isotope multiplexing at ~240 samples/day, boosting protein data points roughly 6.5x over the unlabelled baseline in sensitive (low-input) proteomics.
- Me-Cou N-terminal derivatization de novo sequencing (2026): N-terminal derivatization with N-succinimidyl 7-methoxycoumarin-3-carboxylate (Me-Cou), which boosts low-mass b-ion yield so de novo sequencing can read the N-terminal residues, applied to untargeted peptidomics on LC-TIMS-qTOF/MS.
Papers describing them (6)
- A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry (1999, Proceedings of the National Academy of Sciences, peer-reviewed)
- Derivatization procedures to facilitate de novo sequencing of lysine-terminated tryptic peptides using postsource decay matrix-assisted laser desorption/ionization mass spectrometry (2000, Rapid Communications in Mass Spectrometry, peer-reviewed)
- De Novo Sequencing of Peptides Using Selective 351 nm Ultraviolet Photodissociation Mass Spectrometry (2013, Analytical Chemistry, peer-reviewed)
- PSMtags improve peptide sequencing and throughput in sensitive proteomics (2025, bioRxiv, preprint)
- N-terminal Coumarin Derivatization-aided De Novo Peptide Sequencing and its Application to Peptidomics using LC-trapped Ion Mobility Spectrometry-qTOF/MS (2026, ChemRxiv, preprint)
- N-Terminal Coumarin Derivatization-Aided De Novo Peptide Sequencing and Its Application to Peptidomics Using LC-Trapped Ion Mobility Spectrometry-qTOF/MS (2026, Analytical Chemistry, peer-reviewed)