Ab-seq benchmark (MS antibody sequencing)
benchmark
Ab-seq benchmark (MS antibody sequencing): benchmark. Benchmarks bottom-up MS antibody sequencing over 3,360 runs of six spiked monoclonals, comparing proteases and database versus de novo tools. De novo matched database…
Benchmarks bottom-up MS antibody sequencing over 3,360 runs of six spiked monoclonals, comparing proteases and database versus de novo tools. De novo matched database methods at high concentration, but full de novo antibody reconstruction remained hard.
| Kind | benchmark |
| Acquisition | DDA |
Papers describing it (2)
- Systematic benchmarking of mass spectrometry-based antibody sequencing reveals methodological biases (2024, bioRxiv, preprint)
- Systematic benchmarking of mass spectrometry-based antibody sequencing reveals methodological biases (2025, Cell Systems, peer-reviewed)