Charge derivatization MALDI-PSD de novo sequencing

algorithm · Chemical derivatization assisted

Charge derivatization MALDI-PSD de novo sequencing: algorithm · Chemical derivatization assisted. Charge-derivatizes a proteolytic digest before MALDI post-source decay so the fragment series reads cleanly, evaluated on a phosphoenolpyruvate…

Charge-derivatizes a proteolytic digest before MALDI post-source decay so the fragment series reads cleanly, evaluated on a phosphoenolpyruvate carboxykinase of known structure and then used to sequence an unknown variant from Anaerobiospirillum succiniciproducens.

Kind algorithm
Deep learning no
Acquisition DDA
Family Chemical derivatization assisted

Paper describing it

Authors (6)

Tun-Li Shen, Zhi-Heng Huang, Maris Laivenieks, J. Gregory Zeikus, Douglas A. Gage, John Allison

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