Cricket hydrolysate immunomodulatory peptides
downstream-application
Sequence-resolved workflow for finding immunomodulatory peptides in cricket protein hydrolysate, an alternative-protein food ingredient. The hydrolysate is analysed without a proteolytic digest (PEAKS run with a non-specific enzyme setting) on a Q Exactive, and sequenced de novo with PEAKS to 25,582 assignments at ALC above 50%. Those are filtered to unmodified peptides scoring above 80 that together account for 95% of total abundance (n = 3231), then prioritised by structure-based screening against the STAT3 SH2 domain and molecular dynamics. Selected synthetic peptides reduced LPS-induced IL-6 and TNF-alpha secretion in BV2 microglia, and the hydrolysate itself reduced STAT3 phosphorylation. The paper labels the MS step ‘de novo peptidomics’; its own methods and results call the same operation de novo sequencing, so the phrase denotes de novo sequencing of an undigested peptidome rather than a broader pipeline.
| Kind | downstream-application |
| Deep learning | no |
| Acquisition | DDA |
| Application area | bioactive-peptides |
Papers
- Sequence-resolved discovery and cellular validation of immunomodulatory peptides from cricket protein hydrolysate (2026, Future Foods, peer-reviewed)
- Sequence-Resolved Discovery of Immunomodulatory Peptides from Cricket Protein Hydrolysate as a Functional Alternative Protein Ingredient (2026, SSRN Electronic Journal, preprint)