Bioactive peptides

84 workflows · 2001–2026

Bioactive peptides: Peptides with a function worth finding, from food hydrolysates to amphibian skin secretions. 84 catalogued workflows and 87 papers.

Peptides with a function worth finding, from food hydrolysates to amphibian skin secretions.

Workflows	84
Papers	87
Authors	450
Active	2001-08-01 to 2026-10-08

Workflows (84)

Drosophila immune-induced peptide DIM13 (2001): Determined the full sequence of the N-terminally blocked Drosophila immune-induced molecule DIM13 by de novo interpretation of nano-electrospray multiple-stage MS spectra.
Beta-casein peptic digestion peptides (2007): Follows simulated gastric digestion of bovine beta-casein, identifying peptides by combined de novo interpretation and database search and finding an amino acid variant.
Ranid frog peptide de novo review (2008) (2008): Reviews mass spectrometric methods for de novo sequencing of bioactive skin peptides from ranid frogs, including derivatization and fragmentation choices.
Carnocyclin A circular bacteriocin (2008): Isolates carnocyclin A, a circular bacteriocin from Carnobacterium maltaromaticum, sequenced de novo by tandem MS after enzymatic digestion.
Antimicrobial peptide MS sequencing protocol (2008): A protocol chapter for purifying low-abundance antimicrobial peptides and sequencing them by MALDI and ESI tandem mass spectrometry with de novo interpretation.
Caucasian green frog skin peptides (2008): Sequenced 29 skin secretion peptides of Rana ridibunda de novo from ECD and CID spectra, after oxidation to open disulfide loops.
Collagen IV danger signals in Galleria (2009): Thermolysin-derived collagen IV fragments that stimulate insect immunity in Galleria mellonella were identified by nanoLC-FTICR MS and de novo sequencing, 38 in all.
Baboon theta-defensin sequencing (2010): De novo sequencing by MALDI of two new cyclic theta-defensins purified from hamadryas baboon leukocytes, both active in vitro.
Hyla savignyi tryptophyllin peptides (2010): Spengler group characterized a tryptophyllin-like peptide family from the skin secretion of the Middle East tree frog by manual de novo and composition-based sequencing on FTICR-MS.
Hyla savignyi spacer peptides (2010): Three previously undescribed peptides, including a hydroxyproline-containing one, from the Middle East tree frog skin secretion were sequenced by manual de novo and composition-based sequencing.
Bowman-Birk inhibitor sequencing (2012): Purifies and de novo sequences a novel Bowman-Birk serine protease inhibitor from Lathyrus sativus seeds by electrospray mass spectrometry, a two-headed small protein with distinct reactive loops.
Asian frog skin AMPs (Odorrana) (2012): Identifies eight intact antimicrobial peptides from the skin of the frog Odorrana jingdongensis by de novo sequencing of low-energy ESI CID Q-TOF spectra, supported by Edman degradation and similarity analysis.
Neem leaf tripeptide (2013): Isolates a novel tripeptide from young Azadirachta indica leaves and determines its sequence by de novo MS/MS.
Arca subcrenata antiproliferative peptide H3 (2013): Isolation of an antitumor and antioxidant polypeptide from the ark clam Arca subcrenata, with partial sequences read by MALDI-TOF/TOF de novo sequencing.
Galleria hemolymph danger peptides (2013): Immune-stimulatory peptides from thermolysin-digested Galleria mellonella hemolymph were identified by high-resolution MS using a transcriptome and de novo sequencing, and six were confirmed active.
Arca inflata anti-tumor polypeptide J2-C3 (2013): Isolation of an anti-tumor polypeptide from the clam Arca inflata, with four partial sequences determined by MALDI-TOF/TOF de novo sequencing.
Brevibacillus S-1 cyclic antitumor peptide (2014): Structure of a novel cytotoxic cyclic peptide from a South China Sea Brevibacillus strain was proposed from MALDI-TOF/TOF de novo sequencing.
Dendrobium catenatum antiproliferative peptides (2015): Finds antiproliferative peptides in alcalase hydrolysates of Dendrobium catenatum protein and sequences the most abundant by de novo sequencing.
Tegillarca granosa antitumor protein D2-3 (2015): Purification of an antitumor protein from the blood clam Tegillarca granosa, with six partial amino acid sequences determined by MALDI-TOF/TOF de novo sequencing.
Leuconostoc antifungal peptides (2015): Isolates five novel antifungal peptides from Leuconostoc mesenteroides DU15 sequenced de novo with PEAKS, active against Aspergillus niger.
De novo sequencing of disulphide-rich peptides (Oslo thesis) (2015): Optimised collision energy for QTOF CID of peptides, assessed PEAKS de novo accuracy by peptide length, and de novo sequenced an unknown disulphide-rich bioactive peptide.
Hypsiboas cinerascens cinerascetins (2015): De novo MALDI-TOF/TOF sequencing of skin secretion peptides of the green-tree frog Hypsiboas cinerascens revealed five new hylaseptin-related peptides, cinerascetins, also mapped by MALDI imaging.
Dandelion hydroxyproline glycopeptides (2015): Isolates and de novo sequences ToHyp1 and ToHyp2, two homologous proline-hydroxyproline glycopeptides from Taraxacum officinale flowers that resemble no known protein, and tests their activity.
Odorrana schmackeri calcitonin-like peptides (2015): Determined the complete sequences of two new 34-residue calcitonin-like peptides from Chinese frog skin secretion by de novo MS/MS using CID and ETD, differential alkylation and isotope dimethyl labeling.
American alligator plasma CAMPs (2015): Captured cationic antimicrobial peptides from American alligator plasma with functionalized hydrogel microparticles and sequenced them by de novo-assisted ETD tandem MS, identifying 340 peptides and five novel antimicrobials.
Cowpea hypocholesterolemic peptides (2015): Screens simulated-digestion cowpea peptides for HMG-CoA reductase inhibition, identifying candidates by de novo MS/MS sequencing.
Frog skin cysteine-peptide 3D mass mapping (2015): Screens Odorrana schmackeri and Bombina variegata skin secretions for sulfur-containing peptides via mass defect and isotopic shift, then de novo sequences the candidates to find novel disulfide-bonded peptides.
Rana latastei skin peptidome (2016): Routine de novo sequencing of non-tryptic frog skin peptides on an LTQ Orbitrap Velos with manual spectrum interpretation, on the argument that natural non-tryptic peptides are harder than the tryptic case automation was built for.
Komodo dragon plasma AMPs (2017): Bioprospected Komodo dragon plasma for cationic antimicrobial peptides by ETD de novo sequencing matched to an assembled transcriptome, finding 48 candidates, mostly histone-derived.
Physalaemus nattereri skin peptides (2017): Automated off-line LC MALDI MS/MS of the skin secretion of the Cuyaba dwarf frog Physalaemus nattereri, sequenced de novo in PEAKS and matched by BLAST against amphibian UniProt; most deduced sequences match nothing known.
Walnut iron-chelating peptides (2017): Separates and identifies iron-chelating peptides from defatted walnut flake by nanoLC-ESI-MS/MS and de novo sequencing.
Cowpea peptide transport and cholesterol genes (2018): Identifies cowpea peptides that cross intestinal cell monolayers by de novo sequencing and tests synthetic analogues on cholesterol-related gene expression.
Walnut meal antioxidative peptides (2019): Over a thousand peptides in defatted walnut meal hydrolysate were identified by de novo sequencing, and eight selected peptides showed antioxidant effects on injured nerve cells.
Pajuro (Erythrina edulis) antioxidant peptides (2019): De novo sequencing of alcalase hydrolysate fractions of pajuro protein identified 30 novel peptides, ten of which were synthesised and shown to scavenge radicals.
PepSAVI-MS Amaranthus tricolor antimicrobial peptide (2019): PepSAVI-MS discovered the novel proline-rich antimicrobial peptide Atr-AMP1, whose sequence was determined by EThcD-enabled de novo sequencing with multistage HCD to tell leucine from isoleucine.
Edible bird’s nest peptides (2019): De novo peptide sequencing of edible bird’s nest extracts studied for their neurotrophic properties.
Canidae hepcidin sequence determination (2019): Isolated canine hepcidin from greyhound serum and determined its sequence by manual de novo sequencing, correcting earlier conflicting reports of its size.
Virgin olive oil antihypertensive peptides (2020): Selects 23 virgin olive oil peptides from Orbitrap MS/MS with de novo sequencing and shows ACE inhibition in vitro and antihypertensive effects in rats.
Sea cucumber antioxidative peptides (2020): Compares alcalase against trypsin for releasing antioxidative peptides from Atlantic sea cucumber, sequencing the active peptides de novo by LC-MS/MS and docking them to predict in vivo function.
Greater weever multifunctional peptides (2020): Finds ACE- and DPP4-inhibitory, antioxidant and metal-chelating peptides in greater weever myofibrillar hydrolysates, sequenced de novo.
Ganoderma lucidum antioxidant peptide (2020): Identifies the antioxidant peptide VDLPTCKGF in Ganoderma lucidum hydrolysate by de novo sequencing and tests truncated synthetic variants.
Sea bass muscle bioactive peptidome (2020): Native-peptide discovery workflow combining suspect-screening for short peptides (2-4 aa, including modified aminoacyl derivatives) with database + de novo sequencing for medium-sized peptides (2,665 identified by de novo alone) from sea bass muscle, generalising to any food-matrix bioactive-peptide search.
Neutral-loss screening for di- and tripeptides (2021): A five-minute LC-MS/MS screen keyed on the 46 Da neutral loss of formic acid to find di- and tripeptides in whey and porcine liver hydrolysates, with de novo sequencing to name them.
Sericin antioxidative peptides (2021): De novo sequencing of active fractions of a silk cocoon waste hydrolysate identified seven candidate antioxidative peptides.
Cuban treefrog host defence peptides (2021): Establishes the skin peptidome of the Cuban treefrog Osteopilus septentrionalis by manual de novo sequencing, the host defence peptides in amphibian skin glands being their primary immune armament.
Geotrichum candidum antibacterial tripeptide (2021): Purification and de novo sequencing of a narrow-spectrum bacteriostatic tripeptide from the fungus Geotrichum candidum OMON-1.
Fermented camel milk antimicrobial peptides (2021): RP-HPLC fractionation and LC-MS/MS with PEAKS de novo sequencing identified novel antimicrobial peptides in camel milk fermented with L. plantarum IS10.
Channa striata native peptides (2022): De novo sequencing of the native peptides in an ACE-inhibitory extract of the snakehead fish Channa striata.
ACE2 peptidomimetic binder discovery by AS-MS (2022): Affinity selection from 200-million-member synthetic libraries with de novo MS/MS decoding found ACE2-binding peptides with nanomolar affinity.
Virgin olive oil endogenous peptides (2022): Characterises low molecular weight peptides in virgin olive oil by Orbitrap MS/MS, with several new peptides sequenced de novo by PEAKS and their bioactivity predicted.
FT-MS top-down natural peptide review (2022): Review of FT-MS in the de novo top-down sequencing of natural nontryptic peptides from cones, bees, snakes, amphibians and scorpions.
Frog nontryptic peptide de novo review (2022): Review of mass spectrometric approaches to the de novo sequencing of the nontryptic skin peptides of ranid and hylid frogs.
Tilapia gelatin DPP-IV inhibitory peptides (2022): De novo sequencing of a ginger-protease fish gelatin hydrolysate yielded the DPP-IV inhibitory peptide GPXGPPGPGP.
Arkhangelsk Rana temporaria skin peptides (2023): De novo sequencing of the skin antimicrobial peptides of the Arkhangelsk population of Rana temporaria, tracking how the secreted peptides vary between populations.
Sphaeranthus cyclic peptide (2023): Manual de novo sequencing of antimicrobial and anticancer peptides from the medicinal herb Sphaeranthus amaranthoides, one of them a cyclic peptide.
Foxtail millet α-glucosidase inhibitory peptides (2023): Compares α-glucosidase inhibitory peptides released from raw and cooked foxtail millet prolamins, identifying them by de novo sequencing and screening them virtually.
MS-encoded library hit discovery perspective (2023): A perspective on affinity selection from ultralarge synthetic peptidomimetic libraries decoded by automated de novo MS/MS sequencing rather than barcodes.
Brevibacillus anti-MRSA peptides (2023): Five new antimicrobial peptides purified from Brevibacillus sp. SPR19 were sequenced de novo by tandem MS and the most active one was characterized against MRSA and biofilms.
Agile frog skin defence peptides (2023): Tandem MS de novo sequencing of the skin defence peptides of the central Slovenian agile frog population, on the observation that every species and even population carries a peptidome suited to its habitat.
Saccharomyces pastorianus hydrolysate peptidomics (2023): De novo sequencing in PEAKS Online characterises peptides and candidate bioactive peptides in brewer’s yeast protein hydrolysates.
Pheretima aspergillum anti-asthma peptides (2024): LC-MS/MS de novo sequencing of earthworm (Pheretima aspergillum) decoction and formula granules found bioactive peptides, prioritized by deep learning and docking against the NF-kB complex.
Amphibian skin peptide de novo protocol (2024): Methods in Molecular Biology protocol for the mass spectrometric de novo sequencing of the skin secretion peptides of amphibians, from the Lebedev group.
Olive pomace ACE-inhibitory peptides (2024): Finds low molecular weight peptides with predicted ACE-inhibitory activity in olive pomace, several of them sequenced de novo by PEAKS.
Ovalbumin immunomodulatory peptides (2024): Finds two-way immunomodulatory peptides in ovalbumin hydrolysates prepared with alkaline protease and papain, combining cell assays, de novo sequencing and molecular docking.
EThcD top-down ranid peptide sequencing (2024): Top-down de novo sequencing of intact ranid frog skin peptides by EThcD, giving full sequence coverage including the disulfide ring, and telling leucine from isoleucine, without chemical modification.
Lactobacillus reuteri peptide deep mining (2024): Combines GNPS library search, de novo sequencing and feature-based molecular networking to profile the peptides of Lactobacillus reuteri: 184 peptides, among them four cyclic dipeptides not reported before.
Processed Pinellia ternata bioactive peptides (2024): Molecular networking with de novo sequencing tracks lectin-derived peptides during processing of Pinellia tuber, including ACE inhibitors.
Hirudo thrombin inhibitory peptides (2024): De novo sequencing of a pepsin hydrolysate of leech identified a new thrombin inhibitory peptide.
Sacha inchi digest bioactive peptides (2025): De novo sequencing of simulated-digestion fractions of Sacha inchi press-cake found peptides with alpha-amylase-inhibiting and anti-inflammatory potential.
Arthrospira platensis ACE-inhibitory peptides (2025): Database-assisted identification and de novo sequencing of fractionated thermolysin hydrolysate of spirulina identified the ACE-inhibitory peptides FSESSAPEQHY and ILLYR.
Parallel synthesis + de novo peptide screening (2025): Joint technique pairing de novo sequencing of a simulated-digestion hydrolysate with parallel peptide synthesis, so each predicted sequence is synthesised, screened for activity and checked against the standard’s retention time and MS/MS spectrum. Applied to sea cucumber and seahorse.
Ruditapes decussatus anticancer peptides (2025): De novo sequencing of 135 peptides purified from the edible clam Ruditapes decussatus, 57 of them predicted to have anticancer activity, with fractions cytotoxic to liver and colorectal cancer cell lines.
Lupinus mutabilis iron-chelating peptides (2026): Purified iron-chelating fractions of Andean lupin hydrolysate and identified 176 peptides by de novo sequencing, nine prioritized by docking.
Hellbender skin AMPs (2026): Bioprospects the skin secretions of Cryptobranchus alleganiensis, the hellbender salamander, for antimicrobial peptide candidates. Solid-phase extraction enrichment and Orbitrap Fusion acquisition, PEAKS de novo sequencing of the secretion peptides, then antimicrobial prediction over the de novo sequences. The third GMU host-defence peptide record in the catalog, alongside the red-legged salamander and hemp seed abstracts from the same lab.
Cricket hydrolysate immunomodulatory peptides (2026): Sequence-resolved workflow for finding immunomodulatory peptides in cricket protein hydrolysate, an alternative-protein food ingredient. The hydrolysate is analysed without a proteolytic digest (PEAKS run with a non-specific enzyme setting) on a Q Exactive, and sequenced de novo with PEAKS to 25,582 assignments at ALC above 50%. Those are filtered to unmodified peptides scoring above 80 that together account for 95% of total abundance (n = 3231), then prioritised by structure-based screening against the STAT3 SH2 domain and molecular dynamics. Selected synthetic peptides reduced LPS-induced IL-6 and TNF-alpha secretion in BV2 microglia, and the hydrolysate itself reduced STAT3 phosphorylation. The paper labels the MS step ‘de novo peptidomics’; its own methods and results call the same operation de novo sequencing, so the phrase denotes de novo sequencing of an undigested peptidome rather than a broader pipeline.
Midwife toad skin AMP maturation peptidomics (2026): Compared skin antimicrobial peptide repertoires across life stages of Alytes obstetricans, identifying AMPs from de novo sequences matched to AMP and transcriptome databases.
Chan Pi toad skin anticancer peptides (2026): Peptidomics of Bufo gargarizans toad skin extract by database search and de novo sequencing found about 2500 peptides, and in silico screening yielded a peptide active against TKI-resistant lung cancer cells.
Lupinus mutabilis INFOGEST iron-chelating peptides (2026): Iron-chelating peptides from INFOGEST-digested Andean lupin protein and casein, the lupin ones identified by de novo sequencing and confirmed by synthesis.
Blowfly larval hemolymph AMPs (2026): Bioprospects the hemolymph of Chrysomya megacephala blowfly larvae, which develop in decaying matter, for antimicrobial peptides: larvae are challenged with MRSA, the hemolymph is fractionated by RP-HPLC, active fractions go to QTOF-LCMS, and the spectra are read by de novo sequencing rather than searched, since no database covers this species. The resulting sequence is screened against the APD3, DBAASP, dbAMP and CAMPR4 repositories.
Bacillus amyloliquefaciens BS4 AMPs (2026): Genome mining plus bioactivity-guided purification and de novo sequencing found four antimicrobial peptides, three from the BclA exosporium protein family.
Hemp seed antimicrobial peptides (2026): Screens HAN-J6 hemp seed (Cannabis sativa L.) protein for antimicrobial peptides, a source not previously characterised this way. Defatting, alkaline extraction and acid precipitation, then trypsin/Lys-C digestion and LC-MS; PEAKS 12 de novo sequencing returned 3,985 peptides, and CAMP R3 and AMP Scanner_V2 screened the ALC>=80% list to 56 and 260 candidates respectively, 27 of which both tools agreed on.
Red-legged salamander skin AMPs (2026): Looks for antimicrobial peptides in red-legged salamander skin secretions, where amphibian work has concentrated on frogs and toads and salamander secretions are barely characterised. nanoLC-MS/MS with PEAKS de novo sequencing found 135 unique peptides across four injections, of which CAMP-R3 and AMP Scanner predicted 36 to be antimicrobial.
Asini Corii Colla bioactive peptides (2026): De novo sequences 4764 natural peptides from Asini Corii Colla (donkey-hide gelatin, Ejiao), a traditional Chinese medicine, then screens them in silico (Peptide Ranker, BIOPEP-UWM, docking) down to five candidate dual ACE and DPP-IV inhibitors.
Camel leukocyte peptidome (2026): First proteomic survey of dromedary camel crude leukocyte polypeptides: PEAKS Studio de novo sequencing (ALC 95% or higher) gave 830 peptides beside the 120 proteins from database search, and the de novo peptides were the more strongly enriched for immune-system terms, as a source of candidate bioactive peptides.

Papers (87)

De Novo Sequencing by Nano-Electrospray Multiple-Stage Tandem Mass Spectrometry of An Immune-Induced Peptide of Drosophila Melanogaster (2001, European Journal of Mass Spectrometry, peer-reviewed)
Peptic digestion of β-casein (2007, Journal of Chromatography A, peer-reviewed)
Bioactive peptides from the skin of ranid frogs: modern approaches to the mass spectrometric de novo sequencing (2008, Russian Chemical Bulletin, peer-reviewed)
Isolation and Characterization of Carnocyclin A, a Novel Circular Bacteriocin Produced by Carnobacterium maltaromaticum UAL307 (2008, Applied and Environmental Microbiology, peer-reviewed)
Sequence Analysis of Antimicrobial Peptides by Tandem Mass Spectrometry (2008, Methods in Molecular Biology, peer-reviewed)
De novo sequencing of peptides secreted by the skin glands of the Caucasian Green Frog Rana ridibunda (2008, Rapid Communications in Mass Spectrometry, peer-reviewed)
Identification of collagen IV derived danger/alarm signals in insect immunity by nanoLC-FTICR MS (2009, bchm, peer-reviewed)
De novo sequencing of two new cyclic θ ‐defensins from baboon ( Papio hamadryas ) leukocytes by matrix‐assisted laser desorption/ionization mass spectrometry (2010, Rapid Communications in Mass Spectrometry, peer-reviewed)
Characterization of a peptide family from the skin secretion of the Middle East Tree Frog Hyla savignyi by composition‐based de novo sequencing (2010, Rapid Communications in Mass Spectrometry, peer-reviewed)
Isolation and sequence analysis of peptides from the skin secretion of the Middle East tree frog Hyla savignyi (2010, Analytical and Bioanalytical Chemistry, peer-reviewed)
De novo sequencing and characterization of a novel Bowman–Birk inhibitor from Lathyrus sativus L. seeds by electrospray mass spectrometry (2012, Molecular BioSystems, peer-reviewed)
Antimicrobial peptides from the skin of the Asian frog, Odorrana jingdongensis: De novo sequencing and analysis of tandem mass spectrometry data (2012, Journal of Proteomics, peer-reviewed)
Sequence Determination of a Novel Tripeptide Isolated from the Young Leaves ofAzadirachta indicaA. Juss (2013, International Journal of Peptides, peer-reviewed)
A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata (2013, Marine Drugs, peer-reviewed)
High-Resolution Mass Spectrometry Driven Discovery of Peptidic Danger Signals in Insect Immunity (2013, PLoS ONE, peer-reviewed)
A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata (2013, Marine Drugs, peer-reviewed)
Isolation and Characterization of Marine Brevibacillus sp. S-1 Collected from South China Sea and a Novel Antitumor Peptide Produced by the Strain (2014, PLoS ONE, peer-reviewed)
Antiproliferative effect of Dendrobium catenatum Lindley polypeptides against human liver, gastric and breast cancer cell lines (2015, Food & Function, peer-reviewed)
Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa (2015, Marine Drugs, peer-reviewed)
Novel Antifungal Peptides Produced by Leuconostoc mesenteroides DU15 Effectively Inhibit Growth of Aspergillus niger (2015, Journal of Food Science, peer-reviewed)
De novo sequencing of disulphide rich peptides using tandem mass spectrometry (2015, thesis)
Cinerascetins, New Peptides fromHypsiboas cinerascens: MALDI LIFT-TOF-MS/MSde novoSequence and Imaging Analysis (2015, Journal of the Brazilian Chemical Society, peer-reviewed)
Novel proline-hydroxyproline glycopeptides from the dandelion (Taraxacum officinale Wigg.) flowers: de novo sequencing and biological activity (2015, Plant Science, peer-reviewed)
De novo sequencing of two novel peptides homologous to calcitonin-like peptides, from skin secretion of the Chinese Frog, Odorrana schmackeri (2015, EuPA Open Proteomics, peer-reviewed)
Large Scale Discovery and De Novo-Assisted Sequencing of Cationic Antimicrobial Peptides (CAMPs) by Microparticle Capture and Electron-Transfer Dissociation (ETD) Mass Spectrometry (2015, Journal of Proteome Research, peer-reviewed)
Proteolytic hydrolysis of cowpea proteins is able to release peptides with hypocholesterolemic activity (2015, Food Research International, peer-reviewed)
Screening Method for the Discovery of Potential Bioactive Cysteine-Containing Peptides Using 3D Mass Mapping (2015, Journal of the American Society for Mass Spectrometry, peer-reviewed)
LTQ Orbitrap Velos in routine de novo sequencing of non‐tryptic skin peptides from the frog Rana latastei with traditional and reliable manual spectra interpretation (2016, Rapid Communications in Mass Spectrometry, peer-reviewed)
Discovery of Novel Antimicrobial Peptides from Varanus komodoensis (Komodo Dragon) by Large-Scale Analyses and De-Novo-Assisted Sequencing Using Electron-Transfer Dissociation Mass Spectrometry (2017, Journal of Proteome Research, peer-reviewed)
Analysis of skin derived peptides from the Cuyaba Dwarf Frog Physalaemus nattereri by off-line LC MALDI MS/MS (2017, International Journal of Mass Spectrometry, peer-reviewed)
Separation and identification of iron-chelating peptides from defatted walnut flake by nanoLC-ESI–MS/MS and de novo sequencing (2017, Process Biochemistry, peer-reviewed)
Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells (2018, Food Research International, peer-reviewed)
Antioxidative Effects and Mechanism Study of Bioactive Peptides from Defatted Walnut ( Juglans regia L. ) Meal Hydrolysate (2019, Journal of Agricultural and Food Chemistry, peer-reviewed)
Novel antioxidant peptides obtained by alcalase hydrolysis of Erythrina edulis (pajuro) protein (2019, Journal of the Science of Food and Agriculture, peer-reviewed)
PepSAVI-MS Reveals a Proline-rich Antimicrobial Peptide in Amaranthus tricolor (2019, Journal of Natural Products, peer-reviewed)
Neurotrophic properties and the de novo peptide sequencing of edible bird’s nest extracts (2019, Food Bioscience, peer-reviewed)
Identification of The Canidae Iron Regulatory Hormone Hepcidin (2019, Scientific Reports, peer-reviewed)
Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats (2020, Nutrients, peer-reviewed)
Generation of antioxidative peptides from Atlantic sea cucumber using alcalase versus trypsin: In vitro activity, de novo sequencing, and in silico docking for in vivo function prediction (2020, Food Chemistry, peer-reviewed)
Extraction and characterization of novel multifunctional peptides from Trachinus Draco (greater weever) myofibrillar proteins with ACE/DPP4 inhibitory, antioxidant, and metal chelating activities (2020, Journal of Food Biochemistry, peer-reviewed)
Identification and enhancement of antioxidant P1-peptide isolated from Ganoderma lucidum hydrolysate (2020, Food Biotechnology, peer-reviewed)
Comprehensive identification of native medium-sized and short bioactive peptides in sea bass muscle (2020, Food Chemistry, peer-reviewed)
Tri‐ and dipeptides identification in whey protein and porcine liver protein hydrolysates by fast LC–MS/MS neutral loss screening and de novo sequencing (2021, Journal of Mass Spectrometry, peer-reviewed)
Enzymatic valorization process of yellow cocoon waste for production of antioxidative sericin and fibroin film (2021, Journal of Chemical Technology & Biotechnology, peer-reviewed)
Manual mass spectrometry de novo sequencing of the anionic host defense peptides of the Cuban Treefrog <scp> Osteopilus septentrionalis </scp> (2021, Rapid Communications in Mass Spectrometry, peer-reviewed)
Purification, De Novo Characterization and Antibacterial Properties of a Novel, Narrow-Spectrum Bacteriostatic Tripeptide from Geotrichum candidum OMON-1 (2021, Arabian Journal for Science and Engineering, peer-reviewed)
Novel peptides contribute to the antimicrobial activity of camel milk fermented with Lactobacillus plantarum IS10 (2021, Food Control, peer-reviewed)
Detection of native peptides from Channa striata extract using de novo sequencing (2022, IOP Conference Series: Earth and Environmental Science, peer-reviewed)
Rapid de novo discovery of peptidomimetic affinity reagents for human angiotensin converting enzyme 2 (2022, Communications Chemistry, peer-reviewed)
Characterisation of Endogenous Peptides Present in Virgin Olive Oil (2022, International Journal of Molecular Sciences, peer-reviewed)
FT‐MS in the de novo top‐down sequencing of natural nontryptic peptides (2022, Mass Spectrometry Reviews, peer-reviewed)
Modern Approaches in de novo Sequencing of Nontryptic Peptides of Ranid and Hylid Frogs by Means of Mass Spectrometry: A Review (2022, Journal of Analytical Chemistry, peer-reviewed)
Identification, Screening, and Comprehensive Evaluation of Novel DPP-IV Inhibitory Peptides from the Tilapia Skin Gelatin Hydrolysate Produced Using Ginger Protease (2022, Biomolecules, peer-reviewed)
Mass spectrometry in de novo sequencing of the skin peptides from Arkhangelsk, Russia Rana temporaria: The variability of secreted AMPs in different populations (2023, International Journal of Mass Spectrometry, peer-reviewed)
Biological Activity of Cyclic Peptide Extracted from Sphaeranthus amaranthoides Using De Novo Sequencing Strategy by Mass Spectrometry for Cancer (2023, Biology, peer-reviewed)
Comparison of the generation of α-glucosidase inhibitory peptides derived from prolamins of raw and cooked foxtail millet: In vitro activity, de novo sequencing, and in silico docking (2023, Food Chemistry, peer-reviewed)
Advances in Ultrahigh Throughput Hit Discovery with Tandem Mass Spectrometry Encoded Libraries (2023, Journal of the American Chemical Society, peer-reviewed)
Anti-methicillin-resistant Staphylococcus aureus and antibiofilm activity of new peptides produced by a Brevibacillus strain (2023, PeerJ, peer-reviewed)
Tandem Mass Spectrometry de novo Sequencing of the Skin Defense Peptides of the Central Slovenian Agile Frog Rana dalmatina (2023, Molecules, peer-reviewed)
Functional peptidomics analysis of Saccharomyces pastorianus protein hydrolysates based on different enzyme treatments (2023, Chinese Journal of Chromatography, peer-reviewed)
Identification of the NF-κB Inhibition Peptides in Asthma from Pheretima aspergillum Decoction and Formula Granules using Molecular Docking and Dynamics Simulations (2024, Current Pharmaceutical Analysis, peer-reviewed)
Mass Spectrometric De Novo Sequencing of Natural Peptides (2024, Methods in Molecular Biology, peer-reviewed)
Olive Pomace Extract Contains Low Molecular Weight Peptides and Possesses ACE Inhibitory Activity (2024, International Journal of Molecular Sciences, peer-reviewed)
Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking (2024, Journal of Agricultural and Food Chemistry, peer-reviewed)
EThcD as a Unique Tool for the Top-Down De Novo Sequencing of Intact Natural Ranid Amphibian Peptides (2024, Analytical Chemistry, peer-reviewed)
A Deep Mining Strategy for Peptide Rapid Identification in Lactobacillus reuteri Based on LC-MS/MS Integrated with FBMN and De Novo Sequencing (2024, Metabolites, peer-reviewed)
Food processing drives the toxic lectin reduction and bioactive peptide enhancement in Pinellia ternata (2024, Current Research in Food Science, peer-reviewed)
Identification, screening, and comprehensive evaluation of novel thrombin inhibitory peptides from the hirudo produced using pepsin (2024, Frontiers in Pharmacology, peer-reviewed)
Antidiabetic and Immunomodulatory Properties of Peptide Fractions from Sacha Inchi Oil Press-Cake (2025, Foods, peer-reviewed)
Screening of Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides from Thermolytic Hydrolysate of Arthrospira platensis (2025, Marine Biotechnology, peer-reviewed)
Efficient Screening of Synergistic Antioxidant and AChE Inhibitory Peptides From Sea Cucumber (Stichopus japonicus) Using a Novel Approach Combining De Novo Sequencing and Parallel Peptide Synthesis (2025, Peptide Science, peer-reviewed)
Efficient Screening of α-Glucosidase Inhibitory Peptides From Seahorse Through the Innovative Joint Technique: De Novo Sequencing and Parallel SPOT Synthesis (2025, Journal of Peptide Science, peer-reviewed)
Joint technique “parallel peptide synthesis & de novo sequencing” development for the structure verification and high-throughput activity screening of biological peptides from sea cucumber (Stichopus japonicus) intestinal hydrolysate (2025, Food Research International, peer-reviewed)
Novel peptides from the edible bivalve Ruditapes decussatus target apoptosis, autophagy, and FGF19-FGFR4 signaling in human cancer cell lines (2025, Scientific Reports, peer-reviewed)
Discovery of Iron-Chelating Peptides from Lupinus mutabilis via Integrated Purification and In Silico Validation (2026, Foods, peer-reviewed)
Abstract 4402 De Novo Peptidomics and Bioprospecting Reveal Antimicrobial Peptide Candidates in Cryptobranchus alleganiensis (Hellbender) Skin Secretions (2026, Journal of Biological Chemistry, abstract)
Sequence-Resolved Discovery of Immunomodulatory Peptides from Cricket Protein Hydrolysate as a Functional Alternative Protein Ingredient (2026, SSRN Electronic Journal, preprint)
Early maturation of host antimicrobial peptide defences is associated with host–pathogen coexistence (2026, Nature Chemical Biology, peer-reviewed)
Iron-chelating activity of Andean Lupinus mutabilis Sweet protein and casein under the INFOGEST in vitro gastrointestinal model: peptide identification, molecular docking, and synthesis (2026, Journal of Functional Foods, peer-reviewed)
Identification of Novel Anti- non-small cell lung cancer (NSCLC) Peptide in toad Skin（Chan Pi） Extract through Integrated Peptidomics and in silico Screening (2026, Journal of Pharmaceutical and Biomedical Analysis, peer-reviewed)
From decay to discovery: A new antimicrobial peptide from Chrysomya megacephala (Diptera: Calliphoridae) larvae (2026, Brazilian Journal of Microbiology, peer-reviewed)
Integrated Genome Mining and Bioactivity-Guided Isolation of Antimicrobial Peptides from Bacillus amyloliquefaciens BS4 (2026, Probiotics and Antimicrobial Proteins, peer-reviewed)
Sequence-resolved discovery and cellular validation of immunomodulatory peptides from cricket protein hydrolysate (2026, Future Foods, peer-reviewed)
Identification of Antimicrobial Peptides from HAN-J6 Hemp Seeds Using Mass Spectrometry and De Novo Peptide Sequencing (2026, Journal of Student-Scientists’ Research, abstract)
Discovering Antimicrobial Peptides in Red-Legged Salamander Skin Secretions using De Novo Protein Sequencing (2026, Journal of Student-Scientists’ Research, abstract)
An integrated De novo sequencing and virtual screening strategy for the discovery of low-abundance natural peptides with potential bioactivities from Asini Corii Colla (2026, Journal of Chromatography B, peer-reviewed)
Proteomic profiling of Camelus dromedarius blood leukocytes using protein sequence database searches and de novo peptide sequencing (2026, Journal of King Saud University – Science, peer-reviewed)

Sequencing tools these papers used

Composition-based sequencing, Manual MS/MS de novo interpretation, PEAKS

Authors (450)

A. Yu Kozhevnikov, Aamina Murtza, Abeer Al Hamrashdi, Abiodun Ogunsile, Abraham Espinoza-Culupú, Adebayo Aliyu Lasisi, Afonso D. L. de Souza, Ahmed A. A. Hussein, Alain Van Dorsselaer, Albert J. R. Heck, Albert T. Lebedev, Aldo Laganà, Alethea B. Tabor, Alexandra A. Astafieva, Alexey K. Surin, Alireza Ghassempour, Aliya Al-Ansari, Alvaro Cerda, Amanda E. Cowfer, Amparo I Zavaleta, Ana Aguilar-Galvez, Ana Paula Palacios-Rodriguez, Andrea Cerrato, Andreas Römpp, Andreas Vilcinskas, Andrei Loas, Andrew J. Wommack, Andrés Figueroa-Merma, Andy Wu, Angela Chambery, Angelica P.P. Tonin, Anna Krasilnikova, Anna Laura Capriotti, Annamaria Sandomenico, Anthea Raisis, Anthony J. Quartararo, Antimo Di Maro, Apichart Atipairin, Arton Berisha, Arturo Intiquilla, Atim A. Enyenihi, Augusto Parente, B. Ramachandramurty, Barney Bishop, Belal J. Muhialdin, Benjamin K. Simpson, Benjamin Tapley, Bernhard Spengler, Bingqing Yi, Blanca Hernández-Ledesma, Boran Altincicek, Bradley L. Pentelute, Camila B. Poliseli, Carlos Bloch Jr., Carlos Rodriguez, Carmela Maria Montone, Charin Techapun, Chengfeng Gao, Chengxi Li, Chew Hui Pung, Chiara Cavaliere, Chong Chin Heo, Chris Shaw, Christian E.H. Schmelzer, Christin Stegemann, Christina Nylander, Christine L. Kirkpatrick, Christopher J. Michaels, Christopher Sergeant, Chunyu GAO, Claudio A Alvarez, Cristina Martínez-Villaluenga, Daniel C. Pimenta, Daoshou Qiu, David Campos, David Firer, Dennis Goldfarb, Dewei Shu, Donald Sheppard, Dong Uk Ahn, Dongying Qi, E Chasanah, Ebru Kocadag Kocazorbaz, Edith van der Nol, Eduardo C. Meurer, Eduardo Lopez-Huertas, Edward Bentil, Edward Litton, Ehab El-Dabaa, Elena V. Tsvetkova, Elif Kula, Emily J. Skelly, Emmanuelle Leize, Erwin Torres-Sánchez, Eugene A. Rogozhin, Eugene V. Grishin, Fabiola Almeida Garcia, Fan Zhang, Fanny Guzmán, Fatimah Abu Bakar, Felipe M. A. da Silva, Feng Xie, Fernanda A. Della Rosa, Fernanda C. Martinez, Fernando Y. Abrão, Figen Zihnioglu, Flora Oluwafemi, François Le Mauff, Friedrich Lottspeich, Fulu Pan, Gabriele Rossi, Galina M. Aleshina, Gan Luo, Geisa P.C. Evaristo, Genwei Zhang, Gonçalo M. Rosa, Gregor Torkar, Guodong Zhao, Guopeng Wang, Guru Prasad Srinivasan, Gustavo Guadagnucci Fontanari, Gustavo H.M.F. Souza, Han Wang, Han Zhang, Hang Chen, Haoyuan Zeng, Hassanain Al-Talib, Hector H. F. Koolen, Hemraj Nandanwar, Hend Okasha, Hichem Moulahoum, Hongjiao Chen, Hongwei ZHANG, Hongying Lin, Huan Liu, Hui Ye, Huining Liu, Hussein L. Algboory, Iekhsan Othman, Irina D. Vasileva, Isabella Mathes, Isel Pascual Alonso, J. Miguel Mata, Jaime Bosch, Jesús René Poccohuanca-Gutierrez, Jia Liu, Jia Wu, Jian Xu, Jian-Lin Wu, Jianbing Wang, Jiangling Wan, Jianhua Hao, Jianhua Zhu, Jianping Jiang, Jianyong Sheng, Jie Zhou, Jincai Liang, Jing Zhang (China Academy of Chinese Medical Sciences), Jing Zheng, Jingjing Gao, Jiongyu Liu, Jitang Chen, Joel M. Schnur, Joel P. A. Gummer, Joel Torres Villena, Johanna Rhodes, John C. Vederas, Jonas Bergquist, Jose Maluf, Jose Rubí-Villegas, Joseph S. Brown, José Alfredo Gomes Arêas, Juan Duarte, Juan M. Alcaide-Hidalgo, Juan Pablo Albar, Jue-Liang Hsu, Jumpei Uchiyama, Jun Yan, Junzhong Liu, Kaihua Wei, Kajal Gupta, Kamon Yakul, Kangli Yang, Karim Jiménez‐Aliaga, Kent Vliet, Kersti Karu, Kevin Hopkins, Kevin Quegüe-Montalvo, Khuen Yen Ng, Kieran A. Bates, Kittisak Jantanasakulwong, Klaus Raith, Konstantin A. Artemenko, Krishnendu Mukherjee, Kumaran Subramanian, Lamya Ahmed Al-Keridis, Lanhong Zheng, Lauren Zhong, Leah A. Martin-Visscher, Lee How Lau, Lei Wang (Queen’s University Belfast), Lei Zhang, Lena Gálvez Ranilla, Leslie M. Hicks, Li Zhang, Lifan Li, Lijun Liu, Lili Chen, Lili Zhang, Lilian R. Heil, Linlin HAN, Lisa Smart, Liyan Song, Lourdes Sánchez-Moreno, Lucy Reynell, Luis Javier Gonzalez Lopez, Luis-Felipe Gutiérrez, Luuk N. van Oosten, Lynn M. McMullen, M A Kurnianto, M. Rajeswari Prabha, Maha B. Salem, Mahmoud Abou-Elsoud, Manisha Lnu, Manoj Jangra, Marcelo Gordo, Marcelo H. S. Ramada, Marcelo Rodrigues Marques, Marco J. van Belkum, Maria D. Tolpina, Mariana S. Castro, Mario Hiroyuki Hirata, Markus Langsdorf, Martijn W. H. Pinkse, Martin K. Mead, Masanori Watanabe, Matthew C. Fisher, Maurice Charlet, Megan Devine, Mei Lv, Mei Yeng Yew, Melanie L. Juba, Melissa Claus, Menotti Ruvo, Mervin Pieterse, Mi Sun, Miguel Romero, Min Zhan, Ming Liang, Miriam D. Tolpina, Mohamed ElZallat, Mohamed R. Habib, Mohd Saeed, Monique L. van Hoek, Monthon Lertcanawanichakul, Na Li, Narendran Chiterasu, Nathalie Carte, Nawaf Alshammari, Nazamid Saari, Nicholas C. do Nascimento, Nicole C. Parsley, Noraziah Sahlan, Nukhet Cavusoglu, Nur Maulida Safitri, Nurul Azmiera, Nuttapon Songnaka, Olfat Ali Hammam, Osmindo Rodrigues Pires Júnior, P Martosuyono, Pablo Ramirez, Paul S. Russo, Pawel Michalak, Pedro Ismael Silva Junior, Pengwei Zhang, Peter Bergman, Peter D. E. M. Verhaert, Peter Roepstorff, Philippe Bulet, Phillip A. Jervis, Phisit Seesuriyachan, Pingping Dong, Polonca Trebše, Qi Qin, Qianqian Wang, Qin Zheng, Qiuping Zheng, Qun Shen, Rachele Tamburino, Rafael C. de Araújo, Ralf Hoffmann, Randy M. Whittal, Regasa Chala, Regina Schöps, Reinhard H.H. Neubert, Renate Ulbrich-Hofmann, Rhun Yian Koh, Riccardo Zenezini Chiozzi, Richardson A. Almeida, Robert D. Trengove, Robert I. Lehrer, Robert Settlage, Roberto Alonso Bosch, Rohini Karunakaran, Rokeya S. Rekha, Roman A. Zubarev, Romina Pedreschi, Rongliang Liang, Rongmin Yu, Rosa Nieto, Rosana Chirinos, Rosana Manólio Soares-Freitas, Rosario Dominguez Crespo Hirata, Runkun Zhao, S Budiari, Salma Al-Adwani, Sam Edwards, Samah I. Ghoname, Samir Al Bulushi, Samuel Paterson, Sara Elsa Aita, Sarafadeen Olateju Kareem, Sarah Antilla, Sebastian J. Pomplun, Sebastien Carpentier, Senol Alpat, Sergey A. Kozlov, Sergey V. Kovalev, Sergey V. Ogourtsov, Shabaz Mohammed, Shang Guo, Shariza Sahudin, Shaylyn Scott, Shengfang Gao, Shike Cai, Shilin Gong, Shinji Takenaka, Shouqi Zhang, Shuang Yu, Shuangshuang Lv, Shudong He, Shuze Ren, Sidhika Kannan, Siqi Wang, Sivakumar Shanthirappan, Soi Moi Chye, Somchai Sawatdee, Stephanie Hanna, Stephanie M. Barksdale, Sucheewin Krobthong, Sunday Osaizua Omeike, Susy Piovesana, Swarnalatha Yanamadala, Syafiq Asnawi Zainal Abidin, Syeda Raika Shahid, Sylvie Garneau-Tsodikova, Tam T. Bui, Tanyawat Kaewsalud, Tarun Kumar Upadhyay, Tatiana Yu. Samgina, Tatyana I. Odintsova, Tessa B. Moyer, Thanongsak Chaiyaso, Thomas Defuentes, Thomás Valente de Oliveira, Tianbao Chen, Tianhao Peng, Ting Liu, Tingfei Li, Tito Sánchez-Rojas, Trenton W. J. Garner, Tresai Jordine-Morgan, Tsezi A. Egorov, Tuo Zhang, Túlio O. G. Costa, Valeria Severino, Valquiria M.S. Ribeiro, Vibhavari Aysha Bansal, Vilmar Braz, Vladimir Gorshkov, Vladimir N. Kokryakov, Víctor Izaguirre, Wafa Al-Mamari, Wagner Fontes, Wei Liu, Wei Liu (Huazhong University of Science and Technology), Wenjie Zeng, Wenning Yang, William A. Lefever, Xavier A. Harrison, Xi Huang, Xiangliang Yang, Xianzhang Huang, Xiaoguang Meng, Xiaohui Zhang, Xiaojin Liu, Xiaomei ZHANG, Xiaotong Xiao, Xiaoxin Tu, Xiaoyan Gao, Xiaoyu Chai, Xiaoyu Yang, Xinwen Zhou, Xinxin Li, Xinyu Wang, Xinyue Qi, Xiqing Bian, Xiquan Yin, Xiukun Lin, Xiwang Li, Xue ZHAO, Xuechun Wang, Xuewu Zhang, Xueyan Li, Xuyu Tan, Yajuan Huang, Yang Liu (Beijing University of Chinese Medicine), Yanli Pan, Yao Yi, Yaxiong Liu, Yayang Huang, Yi Zhang, Yilang Zuo, Yimeng Li, Yiming Zhang, Ying Dong, Ying Lv, Yodying Yingchutrakul, Yonggang Liu, Yongxia Fu, Youyou Guo, Yuan Zheng (Yellow Sea Fisheries Research Institute), Yuhao Zhang, Yuliang Zhou, Yunzhen MA, Yutong YAN, Zaiton Hassan, Zayra Vila-Santillán, Zhaowei Meng, Zhen Li, Zhenyu Liu, Zhijun Wu, Zhiyan Chen, Zhuo Cao, Zhuoya Luo, Zirong Yi, Zizhe AN, Zuyue Li, Ákos Végvári, Éric Bonneil

Where the work happened

Australia, Belgium, Brazil, Canada, Chile, China, Colombia, Czechia, Denmark, Egypt, France, Germany, India, Indonesia, Iran, Iraq, Italy, Japan, Malaysia, Netherlands, Nigeria, Peru, Portugal, Russia, Saudi Arabia, Singapore, South Africa, Spain, Sweden, Taiwan, Thailand, Turkey, UK, USA

Seen in the charts

Back to the full map