cryoEM + MS antibody sequencing workflow

adjacent

cryoEM + MS antibody sequencing workflow: adjacent. Workflow combining cryoEM-derived sequences (via ModelAngelo) with LC-MS/MS-based assembly (via Stitch) for simultaneous polyclonal antibody sequencing and epitope…

Workflow combining cryoEM-derived sequences (via ModelAngelo) with LC-MS/MS-based assembly (via Stitch) for simultaneous polyclonal antibody sequencing and epitope mapping. Reports 80–90% accuracy on antibody variable domains and improves MS-based assembly against polyclonal backgrounds.

Kind	adjacent
Acquisition	DDA

Data

What the papers describing this method trained it on and reported its results on, as each paper states it. A version is named only where the paper names one.

Also used, purpose not recorded

• De Novo Sequencing of SARS-CoV-2 and influenza monoclonal antibodies by mass spectrometry using HCD and EThcD fragmentat (as deposited)
• Template-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling (as deposited)

Paper describing it

• Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry (2025, eLife, peer-reviewed)

Authors (4)

Douwe Schulte, Marta Šiborová, Lukas Käll, Joost Snijder

Seen in the charts

• Models and the authors behind them

Back to the full map