Edman-assisted shotgun de novo sequencing
adjacent
Edman-assisted shotgun de novo sequencing: adjacent. Runs successive Edman steps on a tryptic digest and de novo sequences each stage by MS to fix the N-terminal residues of peptides.
Runs successive Edman steps on a tryptic digest and de novo sequences each stage by MS to fix the N-terminal residues of peptides.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
Paper describing it
- Algorithm of Protein Sequence Determination by Combination of the Edman Degradation Method and Shotgun Mass Spectrometry (2018, Biomedical Chemistry: Research and Methods, peer-reviewed)