HTA-protease de novo antibody sequencing
adjacent · Protease strategy
HTA-protease de novo antibody sequencing: adjacent · Protease strategy. Uses two hyperthermoacidic archaeal proteases in place of trypsin or chymotrypsin for de novo antibody sequencing, each yielding about five times more unique peptide…
Uses two hyperthermoacidic archaeal proteases in place of trypsin or chymotrypsin for de novo antibody sequencing, each yielding about five times more unique peptide reads and, with EAciD fragmentation on a ZenoTOF, complete unambiguous sequence coverage of all complementarity-determining regions.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
| Family | Protease strategy |
Paper describing it
- Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry (2026, Cell Systems, peer-reviewed)