Hydrogen atom attachment phosphopeptide sequencing
algorithm · Fragment ion analysis
Hydrogen atom attachment phosphopeptide sequencing: algorithm · Fragment ion analysis. Radical fragmentation by hydrogen atom attachment on a MALDI instrument, which keeps the labile phosphate in place while cleaving the backbone, so tryptic…
Radical fragmentation by hydrogen atom attachment on a MALDI instrument, which keeps the labile phosphate in place while cleaving the backbone, so tryptic phosphopeptides can be sequenced de novo and their phosphosites located.
| Kind | algorithm |
| Deep learning | no |
| Acquisition | DDA |
| Family | Fragment ion analysis |
Paper describing it
- De Novo Sequencing of Tryptic Phosphopeptides Using Matrix-Assisted Laser Desorption/Ionization Based Tandem Mass Spectrometry with Hydrogen Atom Attachment (2018, Analytical Chemistry, peer-reviewed)