Fragment ion analysis
2 methods · 2007–2026
Fragment ion analysis: Work on the fragment ions themselves before any sequence is proposed: charge state, isotope pattern and series assignment, which is what a sequencing algorithm downstream has to assume it already has.
Work on the fragment ions themselves before any sequence is proposed: charge state, isotope pattern and series assignment, which is what a sequencing algorithm downstream has to assume it already has.
The earliest of its 2 methods is PFIA (2007).
| Methods | 2 |
| Papers describing them | 2 |
| Authors | 6 |
| Active | 2007-09-30 to 2026-01-27 |
| Kinds | algorithm (2) |
| Acquisition | DDA (2) |
Methods (2)
Oldest first, by the paper that describes each one.
- PFIA (2007): Peptide Fragment Ion Analyser for interpreting tandem MS data and de novo peptide sequencing.
- Log-transformed mass domain de novo sequencing (2026): Bypasses averagine-based deconvolution by working directly in natural log-transformed m/z space, where charge-state spacing is mass-invariant and therefore supplies an intrinsic internal-calibration reference on both FT-ICR and Orbitrap. Isotopologue pairing in that domain then supports de novo sequencing of intact proteins.
Papers describing them (2)
- Peptide Fragment Ion Analyser (PFIA): a simple and versatile tool for the interpretation of tandem mass spectrometric data and de novo sequencing of peptides (2007, Rapid Communications in Mass Spectrometry, peer-reviewed)
- Mass-Invariant Natural Log-Transformed Mass Spectra Enable Internal Calibration and De Novo Sequencing of Intact Proteins (2026, Analytical Chemistry, peer-reviewed)