MS3 w-ion Leu/Ile discrimination
adjacent · Fragment ion analysis
MS3 w-ion Leu/Ile discrimination: adjacent · Fragment ion analysis. Separates the isomeric residues leucine and isoleucine during de novo sequencing by instrument rather than by inference: multistage ETD isolates the primary odd-electron…
Separates the isomeric residues leucine and isoleucine during de novo sequencing by instrument rather than by inference: multistage ETD isolates the primary odd-electron z-dot ions and HCD then drives radical-site fragmentation into w-ions, whose masses differ between the two isomers and which come out as the most intense peaks in the spectrum. Demonstrated on six natural non-tryptic peptides of 15 to 37 residues from Rana ridibunda frog skin secretion, resolving all 22 Leu/Ile positions.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
| Family | Fragment ion analysis |
Paper describing it
- Discrimination of Leucine and Isoleucine in Peptides Sequencing with Orbitrap Fusion Mass Spectrometer (2014, Analytical Chemistry, peer-reviewed)