N-terminal fragment isolation de novo sequencing
algorithm · Chemical derivatization assisted
N-terminal fragment isolation de novo sequencing: algorithm · Chemical derivatization assisted. Isolates protein N-terminal peptides specifically, through reduction, S-alkylation, guanidination and a cleavable biotin coupling, so several proteins…
Isolates protein N-terminal peptides specifically, through reduction, S-alkylation, guanidination and a cleavable biotin coupling, so several proteins can be N-terminally sequenced in parallel at low picomole level.
| Kind | algorithm |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical derivatization assisted |
Paper describing it
- Specific isolation of N‐terminal fragments from proteins and their high‐fidelity <i>de novo</i> sequencing (2007, Rapid Communications in Mass Spectrometry, peer-reviewed)