Phage display plus de novo mAb reverse engineering
downstream-application
Phage display plus de novo mAb reverse engineering: downstream-application. Combines mass-spectrometry de novo sequencing of antibody variable domains with small phage-displayed libraries and antigen-binding selection to resolve ambiguous CDR…
Combines mass-spectrometry de novo sequencing of antibody variable domains with small phage-displayed libraries and antigen-binding selection to resolve ambiguous CDR residues, recovering functional Fabs for two anti-CD137 antibodies.
| Kind | downstream-application |
| Deep learning | no |
| Acquisition | DDA |
| Application area | Antibodyomics |
Paper describing it
- Combining phage display with de novo protein sequencing for reverse engineering of monoclonal antibodies (2016, mAbs, peer-reviewed)