π-MNovo
algorithm
π-MNovo: algorithm. De novo sequencer adapted to microbial spectra, addressing the fact that existing models were trained largely on non-microbial proteomic data. Built on a purpose-made…
De novo sequencer adapted to microbial spectra, addressing the fact that existing models were trained largely on non-microbial proteomic data. Built on a purpose-made resource of over 10 million annotated spectra from 72 cultured microbial isolates, each digest split into five high-pH reversed-phase fractions for deeper peptide sampling, and paired with evidence-guided candidate selection. Reports 66.30% complete-peptide recall on a seven-species benchmark, 6.9-21.7% relative above four published models, and recovered 1,587 reference-matched peptides from spectra pFind left unidentified.
| Kind | algorithm |
| Deep learning | yes |
| Acquisition | DDA |