Stitch
post-processor · Sequence assembly
Stitch: post-processor · Sequence assembly. Assembles de novo peptides from Casanovo, PEAKS, pNovo and MaxNovo into full antibody sequences, and corrects the two error classes that assembly alone cannot: mass…
Assembles de novo peptides from Casanovo, PEAKS, pNovo and MaxNovo into full antibody sequences, and corrects the two error classes that assembly alone cannot: mass coincidences, where a different residue combination matches the same mass, and I/L ambiguity.
| Kind | post-processor |
| Deep learning | no |
| Acquisition | DDA |
| Family | Sequence assembly |
Code
Live stars, open issues and last-push figures are on the Code activity chart.
Papers describing it (4)
- Template-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling (2022, bioRxiv, preprint)
- Template-Based Assembly of Proteomic Short Reads For De Novo Antibody Sequencing and Repertoire Profiling (2022, Analytical Chemistry, peer-reviewed)
- De novo antibody sequencing based on mass spectrometry (2024, thesis)
- A Handle on Mass Coincidence Errors in De Novo Sequencing of Antibodies by Bottom-up Proteomics (2024, Journal of Proteome Research, peer-reviewed)
Papers using it (2)
Applications and evaluations that ran this method. They are not counted among its authors below.
- Self-assembling proteins compose the chemically resistant shell biomaterial of planktonic tintinnid ciliates (2026, Nature Communications, peer-reviewed)
- Direct Mass Spectrometry-Based Detection and Antibody Sequencing of Monoclonal Gammopathy of Undetermined Significance from Patient Serum: A Case Study (2023, Journal of Proteome Research, peer-reviewed)