Stitch
post-processor · Sequence assembly
Stitch: post-processor · Sequence assembly. Assembles de novo peptides from Casanovo, PEAKS, pNovo and MaxNovo into full antibody sequences, and corrects the two error classes that assembly alone cannot: mass…
Assembles de novo peptides from Casanovo, PEAKS, pNovo and MaxNovo into full antibody sequences, and corrects the two error classes that assembly alone cannot: mass coincidences, where a different residue combination matches the same mass, and I/L ambiguity.
| Kind | post-processor |
| Deep learning | no |
| Acquisition | DDA |
| Family | Sequence assembly |
Code
Live stars, open issues and last-push figures are on the Code activity chart.
Data
What the papers describing this method trained it on and reported its results on, as each paper states it. A version is named only where the paper names one.
Evaluated on
Also used, purpose not recorded
- Anti-FLAG-M2 antibody sequencing (as deposited) · 2022 peer-reviewed, 2022 preprint, 2024 peer-reviewed, 2024 thesis
- Bottom-up data - sequencing (as deposited) · 2022 preprint, 2024 peer-reviewed, 2024 thesis
- Direct mass spectrometry-based detection and antibody sequencing of Monoclonal Gammopathy of Undetermined Significance from patient serum (as deposited) · 2024 thesis
- Sequencing the anti-MUC1 hybridoma antibody 139H2 (as deposited) · 2024 thesis
- Template-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling (as deposited) · 2022 peer-reviewed, 2022 preprint, 2024 thesis
Papers describing it (4)
- Template-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling (2022, bioRxiv, preprint)
- Template-Based Assembly of Proteomic Short Reads For De Novo Antibody Sequencing and Repertoire Profiling (2022, Analytical Chemistry, peer-reviewed)
- De novo antibody sequencing based on mass spectrometry (2024, thesis)
- A Handle on Mass Coincidence Errors in De Novo Sequencing of Antibodies by Bottom-up Proteomics (2024, Journal of Proteome Research, peer-reviewed)
Papers using it (2)
Applications and evaluations that ran this method. They are not counted among its authors below.
- Self-assembling proteins compose the chemically resistant shell biomaterial of planktonic tintinnid ciliates (2026, Nature Communications, peer-reviewed)
- Direct Mass Spectrometry-Based Detection and Antibody Sequencing of Monoclonal Gammopathy of Undetermined Significance from Patient Serum: A Case Study (2023, Journal of Proteome Research, peer-reviewed)