Wastewater microbial + human biomarker metaproteomics
downstream-application
Wastewater microbial + human biomarker metaproteomics: downstream-application. Delft (Pabst / van Loosdrecht) metaproteomics workflow for raw wastewater: efficient protein extraction plus a de novo-sequencing step that customises large public…
Delft (Pabst / van Loosdrecht) metaproteomics workflow for raw wastewater: efficient protein extraction plus a de novo-sequencing step that customises large public sequence databases so search coverage stays high in the presence of a heterogeneous, chemically noisy matrix. Applied to two urban WWTPs over ~3 months to profile a core microbiome (gut bacteria, opportunistic pathogens) alongside ~200 human proteins as population-level health indicators (immunoglobulins, uromodulin, cancer-associated proteins).
| Kind | downstream-application |
| Acquisition | DDA |
| Application area | wastewater-metaproteomics |
Papers
- Wastewater metaproteomics: tracking microbial and human protein biomarkers (2025, ISME Communications, peer-reviewed)