Wastewater metaproteomics
1 workflow · 2025
Wastewater metaproteomics: Wastewater as a population-level sample, carrying both microbial and human peptides. 1 catalogued workflow and 1 paper.
Wastewater as a population-level sample, carrying both microbial and human peptides.
| Workflows | 1 |
| Papers | 1 |
| Authors | 5 |
| Active | 2025-12-18 to 2025-12-18 |
Workflow (1)
- Wastewater microbial + human biomarker metaproteomics (2025): Delft (Pabst / van Loosdrecht) metaproteomics workflow for raw wastewater: efficient protein extraction plus a de novo-sequencing step that customises large public sequence databases so search coverage stays high in the presence of a heterogeneous, chemically noisy matrix. Applied to two urban WWTPs over ~3 months to profile a core microbiome (gut bacteria, opportunistic pathogens) alongside ~200 human proteins as population-level health indicators (immunoglobulins, uromodulin, cancer-associated proteins).
Paper (1)
- Wastewater metaproteomics: tracking microbial and human protein biomarkers (2025, ISME Communications, peer-reviewed)
Where the work happened
Netherlands