A method for antibody sequence identification based on de novo sequencing and homology tag graph theory
CN116779037B · Beijing Institute of Technology · priority 2022
| Patent | CN116779037B ↗ |
| Status | Active |
| Priority date | 2022-07-27 |
| Filed | 2023-07-04 |
| Published (granted) | 2025-08-05 |
| Assignee | Beijing Institute of Technology |
| Also published as | CN116779037A |
Abstract
Translated from Chinese 本发明涉及一种基于从头测序和同源标签图论的抗体序列鉴定方法，属于生物分析技术领域。所述方法首先对待鉴定抗体进行切割，得到的肽段溶液进行液相‑质谱分析；然后通过检索确定轻链、重链恒定区肽段序列，并通过从头测序确定可变区候选肽段序列；之后通过发展动态窗口方法结合物种特异性抗体同源库，对从头测序错误进行有效的识别，并能够区分同分异构体形式的亮氨酸与异亮氨酸，提升了组装准确率与组装稳定性。 The present invention relates to a method for antibody sequence identification based on de novo sequencing and homology tag graph theory, belonging to the field of bioanalysis technology. The method first involves cleaving the antibody to be identified, and subjecting the resulting peptide solution to liquid chromatography-mass spectrometry analysis. The light and heavy chain constant region peptide sequences are then determined through retrieval, and candidate variable region peptide sequences are determined through de novo sequencing. Finally, a dynamic windowing method, combined with a species-specific antibody homology library, is developed to effectively identify de novo sequencing errors and distinguish between isomeric forms of leucine and isoleucine, thereby improving assembly accuracy and stability.
Why it is here
Beijing Institute of Technology: antibody sequence identification from de novo sequencing combined with homology sequence-tag graphs.
Inventors (3)
张永谦, 王旭, 李诺敏
An inventor links to an author page only where the same person is evident, never on a name alone.
Cited by catalogued patents (2)
- CN117607307A A method and its application for de novo sequencing of monoclonal antibodies
- CN121978195A A method for rapidly distinguishing and determining leucine and isoleucine in peptide sequences based on tandem multistage high-resolution mass spectrometry
Cited by (2 patent families, 2 organisations)
- 中国计量科学研究院 academic: CN121978195A
- 深圳市药品检验研究院（深圳市医疗器械检测中心） academic: CN117607307B
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.