A method for rapidly distinguishing and determining leucine and isoleucine in peptide sequences based on tandem multistage high-resolution mass spectrometry
CN121978195A · National Institute of Metrology · priority 2026
| Patent | CN121978195A ↗ |
| Status | Pending |
| Priority date | 2026-03-23 |
| Filed | 2026-03-23 |
| Published | 2026-05-05 |
| Assignee | National Institute of Metrology |
Abstract
The invention relates to the technical field of polypeptide detection, in particular to a method for rapidly distinguishing and determining leucine and isoleucine in a polypeptide sequence based on tandem multistage high-resolution mass spectrometry. The method comprises the steps of (1) carrying out HCD fragmentation on parent ions or child ions of polypeptides to generate imine ions related to leucine and/or isoleucine, (2) carrying out CID fragmentation on the imine ions, detecting relative peak intensities of m/z 44+/-0.2 and m/z 69+/-0.2 generated by fragmentation within a mass axis range of m/z 40-90, and (3) judging the types of leucine and isoleucine in the polypeptides according to the relative peak intensity relation of m/z 44+/-0.2 and m/z 69+/-0.2. The method has higher fragmentation efficiency and confidence level, and provides a basis for rapid and accurate identification of leucine and isoleucine.
Why it is here
National Institute of Metrology, China: telling leucine from isoleucine in a peptide sequence by HCD then CID of their immonium ions.
Inventors (2)
吴佩泽, 李明
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Catalogued papers it cites (1)
- De novo sequencing of peptides by MS/MS (2010) added by the examiner
Matched from the patent’s 1 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- CN116779037B A method for antibody sequence identification based on de novo sequencing and homology tag graph theory