One-Step Free Radical-Initiated Peptide Sequencing Mass Spectrometry Using Negative-ion Mode
KR101500488B1 · Sogang University · priority 2013
| Patent | KR101500488B1 ↗ |
| Status | Expired - Fee Related |
| Priority date | 2013-04-10 |
| Filed | 2013-04-10 |
| Published (granted) | 2015-03-18 |
| Assignee | Sogang University (printed 서강대학교산학협력단) |
| Also published as | KR20140122447A |
Abstract
Translated from Korean 본 발명은 분석하고자 하는 펩타이드를 음이온화 함으로써 단 한번의 충돌 활성화만으로 펩타이드 서열의 동정이 가능한 TEMPO-기반 FRIPS(free radical-initiated peptide sequencing) 방법에 관한 것이다. 본 발명은 단일 충돌 활성화 에너지의 부하를 통해 TEMPO 라디칼의 제거 및 펩타이드 골격 절편화가 모두 수행되어, 긴 duty-cycle을 필요로 하던 기존의 FRIPS 방법의 단점을 훌륭하게 극복함으로써, 효율적인 단백질 구조 분석 방법으로 유용하게 이용될 수 있다. The present invention relates to a TEMPO-based free radical-initiated peptide sequencing (FRIPS) method capable of identifying peptide sequences with only one collisional activation by anionizing the peptide to be analyzed. The present invention overcomes the disadvantages of the conventional FRIPS method which requires a long duty-cycle by performing removal of TEMPO radicals and peptide skeletal fragmentation through a load of single collisional activation energy, Lt; / RTI >
Why it is here
Sogang University: one-step free-radical initiated peptide sequencing in negative-ion mode.
Inventors (2)
Han Bin Oh, 문봉진
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued patents it cites (1)
- US8034623B2 Method for free radical initiated peptide sequencing
Cited by (1 patent families, 1 organisations)
- 서강대학교 산학협력단 academic: KR102582698B1
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.