Method of peptide sequencing by mass-spectrometry and defining of their amino acid sequences
RU2498443C2 · Institute for Analytical Instrumentation · priority 2011
| Patent | RU2498443C2 ↗ |
| Priority date | 2011-08-31 |
| Filed | 2011-08-31 |
| Published (granted) | 2013-11-10 |
| Assignee | Institute for Analytical Instrumentation (printed Федеральное государственное бюджетное учреждение науки Институт аналитического приборостроения Российской академии наук (ИАП РАН)) |
| Also published as | RU2011137344A |
Abstract
FIELD: electricity. SUBSTANCE: method of peptide sequencing by mass-spectrometry and defining of their amino acid sequences is based on fragmentation in mass-spectrometer ion source between the nozzle and skimmer for molecular ions of peptides under the action of controlled electric field and subsequent mass-spectrographic analysis of fragments. Peptide comes to the ion source which electrogasdynamic system allows control of fragmentation degree for molecular ion by means of electric field changing. Further ions are divided in mass analyser and delivered to detector where mass spectrum of the peptide and its fragments with different fragmentation depth in the same spectrum is registered. Mass spectra of the peptide fragments obtained at different values of the electric field force are processes by the recording system, analysed and amino acid sequence is defined for the source peptide in result. Controlled degree of fragmentation in the ion source under action of the varied electrical field within the range of 10 2 -10 4 V/m and residual gas pressure within the range of 100-2000Pa allows determination of amino acid sequence of peptides containing up to 10-15 amino acid residues which corresponds to an average length of peptides, products of protein enzymatic hydrolysis. EFFECT: simplification and speeding up of the system.
Why it is here
Institute for Analytical Instrumentation RAS: peptide sequencing by in-source fragmentation between nozzle and skimmer.
Inventors (6)
Игорь Владимирович Назимов, Николай Васильевич Краснов, Марат Зарифович Мурадымов, Ростислав Анатольевич Бубляев, Михаил Александрович Гаврик, Сергей Сергеевич Присяч
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Catalogued patents it cites (1)
- US6829539B2 Methods for quantification and de novo polypeptide sequencing by mass spectrometry
Cited by catalogued patents (1)
- RU2650639C2 Method of mass-spectrometric sequencing of peptides with the preferential b-ion formation
Cited by (1 patent families, 1 organisations)
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry academic: RU2650639C2
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.