Method of mass-spectrometric sequencing of peptides with the preferential b-ion formation
RU2650639C2 · Institute of Bioorganic Chemistry · priority 2017
| Patent | RU2650639C2 ↗ |
| Priority date | 2017-06-16 |
| Filed | 2017-06-16 |
| Published (granted) | 2018-04-16 |
| Assignee | Institute of Bioorganic Chemistry (printed Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН)) |
| Also published as | RU2017121119A |
Abstract
FIELD: chemistry. SUBSTANCE: invention relates to bioorganic chemistry and can be used to determine the amino acid sequence of peptides and their fragments by mass spectrometry method. For this, sequencing of the dansylated peptide based on the direct introduction of the solution into the ion source followed by fragmentation of the molecular peptide ion in the area between the nozzle and the skimmer of the ion source by the action of an electric field at several discrete values of the electric field strength in the range 10 2 –10 4 V/m and residual gas pressure in the range of 100–2,000 Pa. Presence of a dansyl residue on the N-terminal amino group of the peptide allows to avoid the cyclization of short fragments of the peptide and leads to the preferential formation of b-ions, which are registered predominantly in the form of protonated dehydrated fragments, including the smallest molecular weight ions b 1 and b 2 , which allows to determine the full amino acid sequence of the parent peptide. Average values of the fragmentation parameters: voltage on the capillary 3,400 V, on the nozzle 90–140 V, on the skimmer 60–90 V, the values of these parameters vary depending on the nature and number of amino acids that form the peptide, the system records and determines the amino acid sequence of the peptides. EFFECT: invention provides a simplification of the interpretation of mass spectra and increases the reliability of determining the complete amino acid sequence of the peptides to be studied. 1 cl, 5 dwg, 1 ex
Why it is here
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry: in-source fragmentation sequencing tuned for preferential b-ion formation.
Inventors (2)
Игорь Владимирович Назимов, Ростислав Анатольевич Бубляев
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued patents it cites (2)
- US6829539B2 Methods for quantification and de novo polypeptide sequencing by mass spectrometry
- RU2498443C2 Method of peptide sequencing by mass-spectrometry and defining of their amino acid sequences