Sequencing data-encoded peptides from tandem mass spectra
US12700478B2 · The Hong Kong Polytechnic University · priority 2022
| Patent | US12700478B2 ↗ |
| Status | Active |
| Priority date | 2022-04-11 |
| Filed | 2023-04-10 |
| Published (granted) | 2026-08-04 |
| Assignee | The Hong Kong Polytechnic University (printed Hong Kong Polytechnic University) |
| Also published as | CN116895335A, CN116895335B, EP4261826A1, EP4261826B1, EP4261826C0, US20230326558A1 |
Abstract
Peptide sequencing is important in decoding data stored in a data-encoded peptide. Tandem mass spectrometry (MS/MS) is particularly useful for peptide sequencing. In a computer-implemented method for sequencing the data-encoded peptide from an experimental spectrum, raw data of the experimental spectrum are first preprocessed to remove uninterpretable peaks to yield preprocessed data. A first set of one or more candidate sequences contending for a peptide sequence of the peptide is identified from a spectrum graph. The spectrum graph is formed according to the preprocessed data rather than the raw data for generating a fewer number of candidate sequences to thereby reduce a time cost in sequencing. The first candidate-sequence set is then processed to estimate the peptide sequence to thereby obtain a set of peptide-sequence estimate(s). Each estimate is verified whether it is invalid. The set of peptide-sequence estimate(s) is purged to remove any invalid estimate.
Why it is here
Hong Kong Polytechnic University: de novo sequencing of data-encoded peptides from their tandem spectra, to read stored data back.
Inventors (4)
Zhong-Ping Yao, Cheuk Chi A. Ng, Francis Chung Ming LAU, Wai Man Tam
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued papers it cites (6)
- PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry (2003)
- PepNovo: de novo peptide sequencing via probabilistic network modeling (2005)
- MSNovo: A Dynamic Programming Algorithm for de Novo Peptide Sequencing via Tandem Mass Spectrometry (2007)
- pNovo+: De Novo Peptide Sequencing Using Complementary HCD and ETD Tandem Mass Spectra (2013)
- UniNovo: a universal tool for de novo peptide sequencing (2013)
- Novor: Real-Time Peptide de Novo Sequencing Software (2015)
Matched from the patent’s 8 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- US11694769B2 Systems and methods for de novo peptide sequencing from data-independent acquisition using deep learning