pNovo+: De Novo Peptide Sequencing Using Complementary HCD and ETD Tandem Mass Spectra

peer-reviewed · Journal of Proteome Research · 2013

peer-reviewed · Journal of Proteome Research · 2013. Hao Chi et al. De novo peptide sequencing is the only tool for extracting peptide sequences directly from tandem mass…
Date 2013-02-01
Type peer-reviewed
Venue Journal of Proteome Research
Publisher American Chemical Society
Contribution algorithm
DOI 10.1021/pr3006843
Citations (OpenAlex) 107
Venue 2-year citedness 3.48

Abstract

De novo peptide sequencing is the only tool for extracting peptide sequences directly from tandem mass spectrometry (MS) data without any protein database. However, neither the accuracy nor the efficiency of de novo sequencing has been satisfactory, mainly due to incomplete fragmentation information in experimental spectra. Recent advancement in MS technology has enabled acquisition of higher energy collisional dissociation (HCD) and electron transfer dissociation (ETD) spectra of the same precursor. These spectra contain complementary fragmentation information and can be collected with high resolution and high mass accuracy. Taking these advantages, we have developed a new algorithm called pNovo+, which greatly improves the accuracy and speed of de novo sequencing. On tryptic peptides, 86% of the topmost candidate sequences deduced by pNovo+ from HCD + ETD spectral pairs matched the database search results, and the success rate reached 95% if the top three candidates were included, which was much higher than using only HCD (87%) or only ETD spectra (57%). On Asp-N, Glu-C, or Elastase digested peptides, 69-87% of the HCD + ETD spectral pairs were correctly identified by pNovo+ among the topmost candidates, or 84-95% among the top three. On average, it takes pNovo+ only 0.018 s to extract the sequence from a spectrum or spectral pair on a common personal computer. This is more than three times as fast as other de novo sequencing programs. The increase of speed is mainly due to pDAG, a component algorithm of pNovo+. pDAG finds the k longest paths in a directed acyclic graph without the antisymmetry restriction. We have verified that the antisymmetry restriction is unnecessary for high resolution, high mass accuracy data. The extensive use of HCD and ETD spectral information and the pDAG algorithm make pNovo+ an excellent de novo sequencing tool.

Authors

  1. Hao Chi · Chinese Academy of Sciences, University of Chinese Academy of Sciences
  2. Haifeng Chen · Chinese Academy of Sciences, University of Chinese Academy of Sciences
  3. Kun He · Chinese Academy of Sciences, University of Chinese Academy of Sciences
  4. Long Wu · Chinese Academy of Sciences, University of Chinese Academy of Sciences
  5. Bing Yang · National Institute of Biological Sciences, Beijing
  6. Rui-Xiang Sun · Chinese Academy of Sciences
  7. Jianyun Liu · Beihang University
  8. Wen-Feng Zeng · Chinese Academy of Sciences, University of Chinese Academy of Sciences, Westlake University
  9. Chun-Qing Song · National Institute of Biological Sciences, Beijing
  10. Si-Min He · Chinese Academy of Sciences, University of Chinese Academy of Sciences
  11. Meng-Qiu Dong · National Institute of Biological Sciences, Beijing

Methods and tools

  • pNovo+: Complementary HCD + ETD spectra

Cites (25)

Cited by (27)

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