Database independent detection of isotopically labeled MS/MS spectrum peptide pairs
peer-reviewed · Journal of Chromatography B · 2005
peer-reviewed · Journal of Chromatography B · 2005. Frank Potthast et al. Mass spectrometry data generated in differential profiling of complex protein samples are classically…
| Date | 2005-03-25 |
| Type | peer-reviewed |
| Venue | Journal of Chromatography B |
| Publisher | Elsevier BV |
| Contribution | adjacent |
| DOI | 10.1016/j.jchromb.2004.12.009 |
| Citations (OpenAlex) | 9 |
Abstract
Mass spectrometry data generated in differential profiling of complex protein samples are classically exploited using database searches. In addition, quantitative profiling is performed by various methods, one of them using isotopically coded affinity tags, where one typically uses a light and a heavy tag. Here, we present a new algorithm, ICATcher, which detects pairs of light/heavy peptide MS/MS spectra independent of sequence databases. The method can be used for de novo sequencing and detection of posttranslational modifications. ICATcher is distributed as open source software.
Methods and tools
- ICATcher: Open-source algorithm that finds pairs of light and heavy isotope-tagged MS/MS spectra without a sequence database, as a basis for de novo sequencing and PTM discovery.