Oxidation-guided and collision-induced linearization assists de novo sequencing of thioether macrocyclic peptides
peer-reviewed · Chemical Communications · 2024
peer-reviewed · Chemical Communications · 2024. Ayaka Hayashi et al. Oxidation of a thioether linkage in thioether-closed macrocyclic peptides led to collision-induced…
| Date | 2024-08-05 |
| Type | peer-reviewed |
| Venue | Chemical Communications |
| Publisher | Royal Society of Chemistry (RSC) |
| Contribution | adjacent |
| DOI | 10.1039/d4cc03179b |
| Citations (OpenAlex) | 3 |
Abstract
Oxidation of a thioether linkage in thioether-closed macrocyclic peptides led to collision-induced site-selective linearization of the peptides. This method has allowed for de novo sequencing of thioether macrocyclic peptides. The utility of the sequencing method was demonstrated by identifying the correct peptide sequences from a virtually randomized thioether macrocyclic peptide library.
Methods and tools
- Thioether macrocycle oxidative linearization: Oxidising the thioether of a thioether-closed macrocyclic peptide makes collision-induced dissociation open the ring at one site, so the linearised peptide can be sequenced de novo; demonstrated on a randomised macrocycle library.