Peptide Fragment Ion Analyser (PFIA): a simple and versatile tool for the interpretation of tandem mass spectrometric data and de novo sequencing of peptides

peer-reviewed · Rapid Communications in Mass Spectrometry · 2007

peer-reviewed · Rapid Communications in Mass Spectrometry · 2007. Jagannath Swaminathan et al. The software Peptide Fragment Ion Analyser (PFIA) aids in the analysis and interpretation of tandem mass…
Date 2007-09-30
Type peer-reviewed
Venue Rapid Communications in Mass Spectrometry
Publisher Wiley
Contribution algorithm
DOI 10.1002/rcm.3179
Citations (OpenAlex) 22
Venue 2-year citedness 1.63

Abstract

The software Peptide Fragment Ion Analyser (PFIA) aids in the analysis and interpretation of tandem mass spectrometric data of peptides. The software package has been designed to facilitate the analysis of product ions derived from acyclic and cyclic peptide natural products that possess unusual amino acid residues and are heavily post-translationally modified. The software consists of two programmes: (a) PFIA-I lists the amino acid compositions and their corresponding product ion types for ‘a queried m/z value’ (z = +1) and (b) PFIA-II displays fragmentation pattern diagram(s) and lists all sequence-specific product ion types for the protonated adduct of ‘a queried sequence’. The unique feature of PFIA-II is its ability to handle cyclic peptides. The two programmes used in combination can prove helpful for deriving peak assignments in the de novo sequencing of novel peptides.

Authors

  1. Jagannath Swaminathan · Vellore Institute of Technology
  2. Varatharajan Sabareesh · Indian Institute of Science

Methods and tools

  • PFIA: Peptide Fragment Ion Analyser for interpreting tandem MS data and de novo peptide sequencing.

Cites (4)

Cited by (5)

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