Peptide Sequencing by MALDI 193‐nm Photodissociation TOF MS
peer-reviewed · Methods in Enzymology · 2006
peer-reviewed · Methods in Enzymology · 2006. Joseph W. Morgan et al. Ultraviolet photodissociation time-of-flight (TOF) mass spectrometry (MS) is described as a method for…
| Date | 2006-01-10 |
| Type | peer-reviewed |
| Venue | Methods in Enzymology |
| Publisher | Elsevier |
| Contribution | adjacent |
| DOI | 10.1016/s0076-6879(05)02006-9 |
| Citations (OpenAlex) | 21 |
Abstract
Ultraviolet photodissociation time-of-flight (TOF) mass spectrometry (MS) is described as a method for determination of peptide ion primary structure. Monoisotopic selection and bond-specific activation, combined with the rapidity of TOF MS analysis, render this technique invaluable to the rapidly expanding field of proteomics. Photofragment ion spectra of model peptides acquired using both post-source decay (PSD) focusing and TOF-TOF experimental methods are exhibited. Advantages of 193-nm photodissociation for de novo sequencing of peptide ions are discussed.
Methods and tools
- 193 nm photodissociation MALDI-TOF de novo sequencing: Uses 193 nm ultraviolet photodissociation in MALDI post-source-decay and TOF-TOF instruments to produce peptide fragment spectra suited to de novo sequence determination.