ScopeNovo: a De Novo Peptide Sequencing Method Using Spectrum-Spectrum Similarity to Refine the Prediction Accuracy

peer-reviewed · Bioinformatics · 2026

peer-reviewed · Bioinformatics · 2026. Zhi Song et al. Motivation De novo peptide sequencing from tandem mass spectra is essential for characterizing unknown…
Date 2026-10-15
Type peer-reviewed
Venue Bioinformatics
Contribution post-processor
DOI 10.1093/bioinformatics/btag755
Citations (OpenAlex) 0
Venue 2-year citedness 6.16

Abstract

Motivation De novo peptide sequencing from tandem mass spectra is essential for characterizing unknown proteins, identifying novel post-translational modifications, and analyzing samples without reference databases. While recent deep learning models have improved the predicted peptide accuracy, they typically select the predicted peptide sequence based solely on sequence-level decoding scores. Very often, high scores are assigned to peptides without the direct support from the observed mass spectrum. Results We present ScopeNovo, a de novo peptide sequencing method that improves predicted peptide accuracy by using spectrum-spectrum similarity between the input spectrum and the “representative” spectrum corresponding to the predicted peptide sequence. ScopeNovo generates multiple candidate peptides using an existing peptide de novo sequencing learning model and reranks candidates based on spectrum-spectrum similarity. To test our method, we use π-PrimeNovo as the base learning model. Experimental results on the standard nine-species benchmark datasets V1 and V2 show that ScopeNovo can substantially improve the prediction accuracy. We designed two settings, where Setting 1 is for well-annotated species, and Setting 2 is for species that are not well annotated. For Setting 1, ScopeNovo improves the average peptide accuracies of π-PrimeNovo over the nine species by 6.1% and 4.0% on V1 and V2, respectively. For Setting 2, ScopeNovo can still improve the peptide accuracy of π-PrimeNovo over the nine species by 3.4% and 1.9% on the V1 and V2 benchmark datasets, respectively. Compared to the best known method, RankNovo, the peptide accuracies over the nine species on V1 and V2 of our method for Setting 1 are 0.711 and 0.737, where the peptide accuracies for RankNovo are 0.687 and 0.682. Even for Setting 2, the peptide accuracies of our method are comparable to those of RankNovo. Availability ScopeNovo is implemented in Python under an MIT license. The source code is freely available at https://github.com/SoSongzhi/ScopeNovo.git. The test data are available at https://doi.org/10.5281/zenodo.23181841. Supplementary information Supplementary data are available at Bioinformatics online.

Authors

  1. Zhi Song · City University of Hong Kong
  2. Zejian Ding · City University of Hong Kong
  3. Lusheng Wang · City University of Hong Kong

Methods and tools

  • ScopeNovo: Spectral-library reranking of π-PrimeNovo candidates

Methods it uses

  • Prosit: Deep-learning (bidirectional RNN + attention) predictor of peptide fragment-ion intensities and retention times: widely used to validate and rescore de novo sequencing candidates against predicted spectra.
  • π-PrimeNovo: NAR Transformer (CTC)

Data deposited

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